摘要
探究煤化工烟道气中毒性成分对微藻的影响是利用微藻固定煤化工烟道气CO2实现减排的关键。本文利用不同浓度的NaHS、Na2SO3和NH3·H2O培养Chlorella pyrenoidosa(C.pyrenoidosa),以探究煤化工烟道气主要毒性成分H2S、SO2和NH3气体水溶物的毒性。实验结果表明:NaHS、Na2SO3和NH3·H2O浓度分别低于1mmol/(L·d)、40mmol/(L·d)和7mmol/(L·d)时对C.pyrenoidosa生长无抑制作用,而且Na2SO3[<40mmol/(L·d)]会显著促进C.pyrenoidosa的生长;NaHS添加4mmol/(L·d)时会在生长初期抑制C.pyrenoidosa的生长,NH3·H2O添加35mmol/(L·d)则会直接造成藻细胞的破碎死亡。与对照组相比,NaHS和Na2SO3浓度分别低于1mmol/(L·d)、10mmol/(L·d)时对C.pyrenoidosa的细胞成分无影响;NaHS添加4mmol/(L·d)使藻蛋白含量提高7.13%;Na2SO3添加40mmol/(L·d)使藻蛋白降低13.45%,总糖含量提高42.90%;NH3·H2O的添加会使藻蛋白含量降低,总糖含量提高。微藻生物质整体蛋白质含量较高,可作为蛋白饲料来源。研究结果表明,C.pyrenoidosa对煤化工烟道气中的主要毒性气体有较好的耐受性,利用煤化工烟道气培养微藻具有可行性。
Investigating the resistance of microalgae to harmful gases in coal chemical flue gas is the key to reduce CO2 emission by microalgae fixation.NaHS,Na2SO3,and NH3·H2O were used as the solubles of H2S,SO2,and NH3 to simulate these three typical harmful gases in coal chemical flue gas,and different concentrations were added into the medium of microalgae C.pyrenoidosa to clarify the effects on the physiology and biochemistry of algae cell.Results showed that there were no inhibitory effects on the growth of C.pyrenoidosa when NaHS,Na2SO3 and NH3-H2O were added below 1mmol/(L·d),40mmol/(L·d),and 7mmol/(L·d),respectively.Na2SO3[<40mmol/(L·d)]could significantly promote the growth of C.pyrenoidosa,but NaHS added 4mmol/(L·d)inhibited the growth of C.pyrenoidosa at the initial stage of algae growth,and NH3·H2O added 35mmol/(L·d)directly caused the death of algae cells.Compared with control group,there were no effects on the cell components of C.pyrenoidosa while NaHS and Na2SO3 were added below 1mmol/(L·d)and 10mmol/(L·d),respectively.However,NaHS added 4mmol/(L·d)increased protein content of microalgae by 7.13%.Na2SO3 added 40mmol/(L·d)reduced the protein content by 13.45%,and increased the total sugar content by 42.90%.Overall,microalgae biomass had high protein contents with a potential use of protein feed source.This study demonstrates that C.pyrenoidosa has high tolerance to H2S,SO2 and NH3.Thus,it is feasible to cultivate microalgae using coal chemical flue gas.
作者
刘芬
丰平仲
朱顺妮
王博
王忠铭
LIU Fen;FENG Pingzhong;ZHU Shunni;WANG Bo;WANG Zhongming(Guangzhou Institute of Energy Conversion,Chinese Academy of Sciences,Guangzhou 510640,Guangdong,China;CAS Key Laboratory of Renewable Energy,Guangzhou 510640,Guangdong,China;Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development,Guangzhou 510640,Guangdong,China;University of Chinese Academy of Sciences,Beijing 100049,China)
出处
《化工进展》
EI
CAS
CSCD
北大核心
2020年第11期4668-4676,共9页
Chemical Industry and Engineering Progress
基金
国家重点研发项目(2016YFB0601004)
中泰基金(51861145103)
国家自然科学基金(51861145103)
广东省自然科学基金研究团队项目(2016A030312007)
广东省自然科学基金(2018A030313883)。
关键词
微藻
二氧化碳
煤化工烟道气
毒性
生物质
细胞成分
microalgae
carbon dioxide
coal chemical flue gas
toxicity
biomass
cell components