混合溶液中茶多酚、植酸含量的分光光度法测定及干扰消除

Concentration Determination and Interference Elimination of the Mixed Solution of Tea Polyphenols and Phytic Acid

茶多酚和植酸均可用作食品保鲜剂,复配使用效果更好。采用分光光度法同时测定混合溶液中茶多酚和植酸的含量,并消除相互之间的干扰。研究结果表明,植酸对茶多酚的显色无影响,可采用酒石酸亚铁显色法直接测得混合溶液中茶多酚的含量;而当采用三氯化铁-磺基水杨酸显色时,茶多酚和植酸均有显色,且混合溶液中茶多酚质量浓度影响植酸的显色能力。采用数学模拟法可消除茶多酚对植酸显色能力的干扰,获得植酸的标准曲线。此方法检测最佳范围为茶多酚质量浓度0~0.300 mg/mL,植酸质量浓度0~3.000 mg/mL,回收率96%~103%。方法操作简单,准确度高,可为混合组分的同时测定提供理论依据。

Tea polyphenols and phytic acid both can be used as food preservative.In this paper,the mixed solutions concentrations of tea polyphenols and phytic acid were simultaneously determined by spectrophotometr,and the interference was eliminated.The results showed that phytic acid had no effect on the coloration of tea polyphenols,and the concentration of tea polyphenols in the mixed solution could be determined immediately by ferrous tartrate coloration method.Tea polyphenols and phytic acid both had color development when the polyferric chloride-sulfosalicylic acid was a chromogenic agent,and the color development ability of phytic acid were affected by the mass concentration of tea polyphenols in the mixed solution.The interference of tea polyphenols on the color development ability of phytic acid could be eliminated by the mathematical simulation method,and the standard curve of phytic acid were obtained.By this method,the optimal determination range was the tea polyphenol mass concentration of 0-0.300 mg/mL,the phytic acid mass concentration of 0-3.000 mg/mL,and the recovery rate of 96%-103%.The method is simple to operate and has high accuracy,and it can provide a theoretical basis for simultaneous determination of mixed components.