大豆滞绿(stay-green)突变体诱变后代生理指标及品质分析

Analysis of physiological indexes and quality in mutant progeny of soybean stay-green mutants

为研究大豆滞绿突变体后代中生理指标和品质性状的差异,利用经60 Co-γ诱变的大豆滞绿突变体‘Z-94320’的诱变后代M6为试材,对后代不同种皮色不同子叶色大豆的各时期叶绿素含量、鼓粒期光合作用以及成熟期蛋白质和脂肪含量进行测定并分析。结果表明:滞绿型后代的叶绿素SPAD平均最高可达41.76±0.93,平均变化率可达(12.27±0.70)%,较普通型后代在衰老时期会出现明显的延迟;绿色种皮后代植株叶片的气孔导度会受到抑制,但各类群之间的光合参数无显著差异;滞绿型后代较普通型后代会出现显著的品质提升,且叶绿素变化率与蛋白质含量、脂肪含量呈现极显著的正相关,滞绿型类群中浅绿色种皮、绿色子叶大豆的蛋白质含量和脂肪含量比绿色种皮、子叶大豆分别高出(44.35±0.16)和(21.57±0.03)g/100g,因此,品质更优。

In order to study the differences of physiological indexes and quality characters in the progenies of soybean green retarded mutant,the mutagenic progenies M6 of soybean stay-green mutant‘Z-94320’induced by 60 Co-γwere used as test materials to measure and analyze the chlorophyll content in different stages,photosynthesis during the seed-filling period,protein and fat of soybeans with different seed coat color and cotyledon color.The results showed that the average content of chlorophyll of stay-green progenies in the senescence stage was 41.76±0.93,and the average rate of change was(12.27±0.70)%,which was significantly higher than that of the common progenies.The stomatal conductance of green seed coat progeny was inhibited,but there was no significant difference in photosynthetic parameters among different groups.The quality of the stay-green progenies was significantly higher than that of the common progenies,and the change rate of chlorophyll had a extremely significant positive correlation with protein content and fat content.In stay-green group,the protein content and fat content of light green seed coat and green cotyledon soybean were(44.35±0.16)and(21.57±0.03)g/100 g higher than those of green seed coat and cotyledon soybean,respectively.