水稻BBX基因家族生物信息学及表达模式分析

Bioinformatics and Expression Pattern Analysis of BBX Gene Family in Rice

BBX家族是植物中一类重要的光温响应转录因子,在调节植物生长发育、光形态建成以及响应环境逆境等方面发挥重要作用。为揭示该家族相关分子特征以及表达模式,本研究采用生物信息学与转录组学相结合的方法,对水稻BBX基因结构、蛋白基序以及响应激素与非生物逆境的表达模式进行了系统研究。基因结构分析发现该家族基因整体长度较小,大部分在4 kb以内;蛋白氨基酸序列分析发现主要含有6种氨基酸基序类型,其中氨基酸基序2是构成该家族的基本基序。通过组织时空表达分析发现,该家族表现出较强的组织特异性和光周期性;通过基因芯片激素处理的数据分析,水稻BBX家族的表达水平在整体上对脱落酸(ABA)和茉莉酸(JA)处理下表达变化显著,在赤霉素(GA)处理下表达差异不显著;通过高温、干旱、盐等逆境处理后的转录组测序分析,发现该家族多数成员对逆境的响应变化差异不大,少数基因表达差异显著,具有多样化和特异性。综上表明,水稻BBX家族基因组层面的分子特征具有相似性和保守性,响应激素和逆境的表达水平多样化,暗示该家族可能参与了多种逆境响应途径。

The BBX family is an important light-temperature response transcription factor in plants, which plays an important role in regulating plant growth and development, photomorphogenesis and response to environmental stress. In order to reveal the molecular characteristics and expression patterns of this family, we used a combination of bioinformatics and transcriptomics to systematically study the expression patterns of rice BBX gene, protein motifs and response hormones and abiotic stresses. The gene structure analysis revealed that the family gene was small in length, most of which was within 4 kb. The amino acid sequence analysis revealed that there were mainly six amino acid motif types, of which amino acid motif 2 was the basic motif of the family.Through tissue spatiotemporal expression analysis, the family showed strong tissue specificity and photoperiod.Through the expression analysis under hormone treatment, the expression level of rice BBX family was significantly changed under the treatment of abscisic acid(ABA) and jasmonic acid(JA), but not for gibberellin(GA) treatment. Through transcriptome sequencing analysis, most members of the family had little difference in response to abiotic stresses. A small number of gene expression differences are significant, diverse and specific.Studies have shown that the molecular characteristics of the BBX family genome in rice are similar and conservative, and the level of response to adversity and hormones is diverse, suggesting that the family may be involved in a variety of signal pathways.