绿豆AP2/ERF转录因子家族的生物信息学鉴定与特征分析

Bioinformatics Identification and Characteristic Analysis of AP2/ERF Transcription Factor in Mung bean

AP2/ERF转录因子是一种在植物生长发育和(非)生物胁迫应答等过程中发挥重要作用的转录因子家族。本研究在绿豆全基因组范围内,利用生物信息学方法分析了AP2/ERF转录因子家族的成员、结构功能和理化性质等信息。结果表明:绿豆AP2/ERF转录因子家族包含87个成员,划分为ERF、DREB、AP2和RAV亚家族,其中76个成员不均匀的分布在11条染色体上;不同亚家族基因结构差异较大,除高度保守的AP2/ERF结构域外还存在其它保守结构域;各成员的蛋白分子量介于13.99~75.40 kD,理论等电点介于4.87~9.72,可能不存在信号肽且均属于亲水性蛋白;α-螺旋和无规则卷曲是主要的二级结构元件,三级结构与二级结构预测结果基本一致;丝氨酸、苏氨酸和酪氨酸是主要的磷酸化作用位点,不同成员具有不同数量和长度的固有无序化区域。本研究结果将为进一步研究绿豆AP2/ERF转录因子功能提供理论依据,同时也为绿豆品种抗逆改良提供了依据。

The AP2/ERF transcription factor(TF) is a huge gene family which plays an important role in plant growth and(non-) biotic stress responses in plant. In this study, a complete overview of AP2/ERF TFs including the members, genic structure, biological function as well as physicochemical properties was analyzed using bioinformatics methods in mung bean. The results showed that 87 AP2/ERF TFs were obtained from mung bean, which were divided into ERF, DREB, AP2 and RAV subfamilies, 76 of which were unevenly distributed on 11 chromosomes;the genic structures of different subfamilies were quite different, there were other conserved domains besides the highly conserved AP2/ERF domain;the molecular weight of each TF protein ranged from 13.99 kD to 75.40 kD,the theoretical isoelectric point was between 4.87 and 9.72, which might not have signal peptides and were both hydrophilic protein;The α-helix and random coil were the main secondary structural elements, and the prediction results of tertiary structure were basically consistent with that of the secondary structure;the serine, threonine and tyrosine were the main phosphorylation action sites, and different TFs also had intrinsically disordered regions with different numbers and lengths. The results of this study will provide a theoretical foundation for further study on the function of AP2/ERF TFs in mung bean, and also will provide the basis for the resistant improvement of mung bean varieties.