暗培养条件下水稻苗期第一节间长度的QTL定位

QTL Mapping of the First Internode Length in Rice at Seedling Stage under Dark Condition

中胚轴及第一节间长的水稻顶土能力强,易出苗。为了进一步发掘和利用中胚轴和第一节间长的水稻种质资源,本研究利用来自云南的地方品种Ch5-10 (水稻,偏籼性)/Ch6-11 (陆稻,偏粳性)组配的RIL9群体及特青(籼稻)/星青叶(粳稻)组配的F2群体分别构建遗传连锁图谱,并进行了暗培养条件下第一节间长度的QTL定位。Ch5-10/Ch6-11组配的RIL9群体共检测到3个QTLs,分别位于第1和第6染色体上。其中qFIL-1的效应最大,贡献率为17%,表现为来自亲本Ch6-11的等位基因增加第一节间长度;特青/星青叶组配的F2群体仅检测到1个QTL:qFIL-2,位于第2染色体上,贡献率为9%,表现为来自亲本星青叶的等位基因增加第一节间长度,同时该位点检测到加性×加性上位性效应。

Emergence rate of long mesocotyl and first internode length germplasm was relatively higher and have strong top soil ability. In order to further explore and utilize the germplasm resources of hypocotyl and the first internode. An F9 recombinant inbred lines(RILs) population derived from a cross of Ch5-10 and Ch6-11 and an F2 population derived from a cross between Teqing(female parent, indica type) and Hoshiaoba(male parent,japonica type) were used in this study. Ch5-10(female parent, lowland line, indica type) and Ch6-11(male parent,upland line, japonica type) were local varieties from Yunnan province.The genetic linkage maps were constructed using the RIL9 population and the F2 population, respectively. And then,The two populations were used for detecting QTLs for the first internode length at seedling stage under dark condition. In the RIL9 population, three QTLs for the first internode length were identified on chromosomes 1 and 6. Among them, qFIL-1 had largest effect which explained 17% phenotypic variation, and the allele from Ch6-11 increased the first internode length.In the F2 population, only one QTL for the first internode length was identified on chromosomes 2, and qFIL-2 explained 9% phenotypic variation and the allele from Hoshiaoba increased the first internode length. An additive×additive epistatic QTL were found in the F2 population.