摘要
目的:考察龙葵生物碱对结肠癌DLD-1细胞增殖及凋亡的影响。方法:采用CCK8实验、AO-EB双染色法和流式细胞仪检测法,考察龙葵生物碱对结肠癌DLD-1细胞增殖及凋亡的影响。结果:CCK-8实验显示龙葵生物碱对DLD-1细胞增殖具有抑制作用,其半数抑制浓度(IC50)为0.94 mg/mL;AO-EB双染色荧光显色法实验显示不同浓度的龙葵生物碱均能诱导DLD-1细胞凋亡;流式细胞法实验结果显示不同浓度的龙葵生物碱均呈现诱导细胞凋亡的作用。结论:龙葵生物碱在一定程度上对DLD-1细胞增殖呈抑制作用,同时可诱导细胞凋亡。
Objective:To investigate the effects of alkaloids from Solanum nigrum on proliferation and apoptosis of colon cancer DLD-1 cells.Method:The effects of alkaloids from Solanum nigrum on the proliferation and apoptosis of DLD-1 cells were investigated by CCK8,AO-EB double staining and flow cytometry.Result:CCK-8 assay showed that alkaloids from Solanum nigrum inhibited the proliferation of DLD-1 cells,and its half inhibitory concentration(IC50)was 0.94 mg/mL;AO-EB double staining fluorescence staining showed that different concentrations of alkaloids from Solanum nigrum induced apoptosis of DLD-1 cells;Flow cytometry showed that different concentrations of Solanum nigrum alkaloids could induce apoptosis.Conclusion:Solanum nigrum alkaloids can inhibit the proliferation of DLD-1 cells and induce apoptosis to some extent.
作者
周晓
靳贝贝
樊东升
段秀俊
胥鹏鹏
Zhou Xiao;Jin Beibei;Fan Dongsheng;Duan Xiujun;Xu Pengpeng(Shanxi University of Traditional Chinese Medicine,Jinzhong Shanxi 030619;Shanxi Hospital of Integrated Traditional and Western Medicine,Taiyuan Shanxi 030006)
出处
《山西中医药大学学报》
2020年第5期328-330,334,共4页
Journal of Shanxi University of Chinese Medicine
关键词
龙葵生物碱
结肠癌
DLD-1细胞
增殖
凋亡
Solanum nigrum alkaloids
colon cancer
DLD-1 cells
proliferation
apoptosis