摘要
目的观察低氧条件下肺泡上皮细胞对肺动脉平滑肌细胞(PASMCs)的作用。方法首先,低氧条件下培养肺泡上皮细胞48 h,采用WB法检测细胞中CX3CL1蛋白的表达,ELISA法检测细胞上清液中CX3CL1的浓度;其次,采用Transwell小室共培养肺泡上皮细胞和PASMCs 48 h,采用MTT法检测PASMCs增殖;最后利用siRNA沉默PASMCs的CX3CR1,采用MTT法检测PASMCs增殖。结果与对照组比较,肺泡上皮细胞在低氧处理48 h后,CX3CL1蛋白表达、细胞上清液中CX3CL1浓度明显增加(P<0.05);与对照组相比,PASMCs与肺泡上皮细胞共培养48 h后,PASMCs增殖明显增加(P<0.05);siRNA沉默后的PASMCs与大鼠肺泡上皮细胞共培养48 h后,PASMCs增殖无明显增加(P>0.05)。结论低氧条件下肺泡上皮细胞通过CX3CL1促进PASMCs增殖。
Objective To investigate the effect of pulmonary alveolar epithelial cells(AECs)on pulmonary artery smooth cells(PASMCs)under hypoxic conditions.Methods First,under hypoxic conditions,rat AECs were cultured for 48 h,and the expression of CX3CL1 protein in the cells was detected by WB,while the concentration of CX3CL1 in the cell supernatant was detected by ELISA.Second,Transwell compartment co-cultured rat AECs and PASMCs for 48 h.MTT assay was used to detect the proliferation of PASMCs.Finally,siRNA was used to silence CX3CR1 PASMCs,and MTT method was used to detect the proliferation of PASMCs.Results Compared with the control group,the expression of CX3CL1 protein and the concentration of CX3CL1 in cell supernatant increased significantly after hypoxic treatment for 48 h in rat AECs(P<0.05).Compared with the control group,the proliferation of PASMCs increased significantly after 48 h co-culture of AECs and PASMCs(P<0.05).After 48 h of co-culture of PASMCs and rat AECs after siRNA silencing,there was no significant increase in the proliferation of PASMCs(P>0.05).Conclusion Hypoxia-induced AECs CX3CL1 triggers lung smooth proliferative expansion.
作者
魏冠平
张淼
黄煜
何庆
WEI Guanping;ZHANG Miao;HUANG Yu;HE Qing(Southwest Jiaotong University College of Medicine,Chengdu 610036,Sichuan,China;The Affiliated Hospital of Southwest Jiaotong University,The Third People's Hospital of Chengdu,Chengdu 610036,Sichuan,China)
出处
《心血管病学进展》
CAS
2020年第10期1106-1110,共5页
Advances in Cardiovascular Diseases
基金
国家自然科学基金青年基金(81501640)。
关键词
低氧
CX3CL1
肺泡上皮细胞
肺动脉平滑肌细胞
Hypoxia
CX3CL1
Pulmonary alveolar epithelial cells
Pulmonary artery smooth muscle cells