孤独症谱系障碍儿童维生素D与水溶性维生素水平分析

Analysis of vitamin D and water-soluble vitamin levels in children with autism spectrum disorder

目的探讨孤独症谱系障碍(ASD)与血维生素D及水溶性维生素水平的关系。方法选取2019年1月至12月在本院儿童康复科门诊就诊及进行康复训练的ASD患儿71例为ASD组,同期抽取在我院门诊行常规体检与ASD组性别分布相匹配的71例儿童为对照组。采用化学发光法检测血清25(OH)D3水平,采用超高效液相色谱-串联质谱法测定血水溶性维生素的含量。结果ASD儿童血25(OH)D3、维生素B1、维生素B5、维生素B6、维生素B9含量低于对照组(P<0.05),两组血维生素B2、维生素B3、维生素B7、维生素B12及维生素C含量无统计学差异(P>0.05);不同维生素D水平的ASD儿童血液中维生素B5、维生素B6、维生素B9有统计学差异(P<0.05),维生素B1水平无统计学差异(P>0.05);维生素D水平与维生素B9呈显著正相关(P<0.05),与维生素B1、维生素B5、维生素B6相关性分析无统计学意义(P>0.05)。结论ASD儿童血维生素D、维生素B1、维生素B5、维生素B6及维生素B9水平降低,维生素B5、维生素B6、维生素B9在不同程度的维生素D水平的ASD儿童中存在差异,且血中维生素D水平与维生素B9水平呈显著正相关关系,提示ASD儿童需要监测维生素D及水溶性维生素水平并根据情况补充。

Objective To investigate the relationship between autism spectrum disorders and blood levels of Vitamin D and water-soluble vitamins.Methods A total of 71 children with autism spectrum disorder who attended the outpatient clinic of the Children's Rehabilitation Department of our hospital and received rehabilitation training from January to December 2019 were recruited as ASD group.Age-and gender-matched children undergoing routine physical examination in our hospital were recruited as control group.Their serum 25(OH)D3 level was determined by chemiluminescence method.The concentration of water-soluble vitamins in blood was determined by ultra-high performance liquid chromatography-tandem mass spectrometry.Results The blood levels of 25(OH)D3,Vitamin B1,Vitamin B5,Vitamin B6,and Vitamin B9 in children with ASD were lower than those in the control group(P<0.05).No statistical difference was observed in Vitamin B2,Vitamin B3,Vitamin B7,Vitamin B12 or Vitamin C between the two groups(P>0.05).There were statistical differences in Vitamin B5,Vitamin B6,and Vitamin B9 in the blood of ASD children with different Vitamin D levels(P<0.05),but no statistical difference in Vitamin B1 levels(P>0.05).The level of Vitamin D was significantly positively correlated with Vitamin B9(P<0.05).No statistical correlation was observed between Vitamin D and Vitamin B1,Vitamin B5 or Vitamin B6(P>0.05).Conclusion The blood levels of Vitamin D,Vitamin B1,Vitamin B5,Vitamin B6 and Vitamin B9 in children with autism spectrum disorder are reduced.The levels of Vitamin B5,Vitamin B6,and Vitamin B9 are different in children with autism spectrum disorder with different levels of Vitamin D,and there is a significant positive correlation between the level of Vitamin D in the blood and the level of Vitamin B9.These results suggest that the children with autism spectrum disorder should have their levels of Vitamin D and water-soluble Vitamins monitored and take some vitamins according to the condition.