耐受高浓度甲醛真菌的筛选及鉴定

Screening and Identification of High Concentration Formaldehyde-Tolerant Fungi

生活环境中的甲醛污染对人体健康存在着严重威胁,利用微生物分解甲醛的相关研究备受关注。通过筛选,获得10株耐受甲醛真菌,其耐受的甲醛质量浓度为1.2~1.9 g/L。通过形态鉴定,10株真菌分别隶属于曲霉属Penicillium和青霉属Aspergillus。扩增耐受能力最强的4株真菌的ITS和β-tubulin部分序列,构建系统发育树,确定3株青霉(HFTS2、HFTS8和HFTS10)同属于产红青霉P.rubens,1株曲霉(HFTS5)为众多曲霉A.creber。本研究首次发现产红青霉和众多曲霉具有高浓度甲醛耐受性,为进一步探索真菌甲醛代谢和研发工程真菌奠定基础。

Formaldehyde pollution in living environment can seriously threaten the human body.Using microorganisms to decompose formaldehyde has attracted more attention.In order to identify formaldehyde-tolerant fungi,we identified 10 fungal strains that were highly resistant to formaldehyde from a living fungal library,named as HFTS1-HFTS10.The formaldehyde tolerance concentration was from 1.2 to 1.9 g/L,and belonged to the genera Aspergillus and Penicillium,respectively.Then phylogenetic trees of the dataset of ITS andβ-tubulin sequences were constructed,and the results showed that these three strains(HFTS2,HFTS8 and HFTS10)were identified as Penicillium rubens,and HFTS5 strain was identified as Aspergillus creber.Both P.rubens and A.creber were firstly reported to exhibit high concentration formaldehyde tolerance.This research lays a foundation for further study on formaldehyde metabolism and engineering fungi.