基于磁共振成像研究侯氏黑散对脑缺血大鼠神经血管功能恢复的作用

Effects of Houshiheisan Formula on the recovery of neurovascular function in rats with cerebral ischemia based on MRI

目的利用磁共振成像技术研究侯氏黑散对脑缺血大鼠神经血管功能的影响。方法采用大脑中动脉线栓法建立大鼠永久性脑缺血模型。雄性SD大鼠随机分为假手术组、模型组、侯氏黑散低剂量组(5.25 g/kg)组、侯氏黑散高剂量组(10.50 g/kg)组和银杏叶提取物组(28 mg/kg)。神经功能评分评价各组大鼠神经功能缺损程度;T2加权成像和T2弛豫成像测量脑缺血大鼠脑梗死体积和脑组织损伤程度;动脉自旋标记灌注加权成像和磁共振血管成像评价脑缺血大鼠颅内血管的血流动力学的改变。结果与模型组相比,侯氏黑散高剂量组与银杏叶提取物组神经功能评分在脑缺血后第5、7天降低(P<0.05)。与模型组相比,各给药组脑梗死体积缩小,脑白质和灰质损伤减轻(P<0.05),缺血半球脑血流量和血管信号强度增加(P<0.05)。与假手术组相比,模型组缺血半球脑血流量和血管信号强度降低(P<0.01)。结论侯氏黑散可以减轻脑缺血大鼠脑损伤和改善颅内血流动力学,起到对神经血管功能的保护和恢复作用。

Objective To non-invasively assess the therapeutic effect of Houshiheisan(HSHS)Formula on the recovery of neurovascular function after cerebral ischemia in rats with application of magnetic resonance imaging(MRI).Methods Focal cerebral ischemia model was induced by permanent middle cerebral artery occlusion(pMCAO)in male sprague-Dawley(SD)rats.Then the rats were randomly divided into 5 groups:sham group,model group,HSHS low-dose group(5.25 g/kg),HSHS high-dose group(10.50 g/kg)and Ginkgo biloba extract group(28 mg/kg).Nurological score was assessed after surgery to evaluate the degree of nervous function defects.T2 weighted imaging(T2 WI)and T2 relaxation imaging were used to measure the volume of cerebral infarction and the degree of brain tissue damage in rats with cerebral ischemia.Arterial spin labeled perfusion weighted imaging(ASL-PWI)and magnetic resonance angiography(MRA)were used to evaluate the hemodynamic changes of intracranial vessels.Results Compared with the model group,HSHS high-dose group and Ginkgo biloba extract group decrease neurological function score on the 5 th and 7 th day(P<0.05).Compared with the model group,the infarct volume reduced and the damage of white matter and gray matter was decreased,and the cerebral blood flow and signal intensity of ischemic hemisphere increased in all treatment groups(P<0.05).Compared with the sham group,the cerebral blood flow and signal intensity of ischemic hemisphere decreased in the model group(P<0.01).Conclusion HSHS seems to alleviate brain injury and improve the intracranial hemodynamics,and promote the recovery of neurovascular function.