天南星和虎掌南星对大鼠水液代谢和血液流变学的影响

Effect of Arisaema Rhizoma and Pinelliae Pedatisectae Rhizoma on Water Metabolism and Hemorheology in Rats

目的:观察天南星与虎掌南星对大鼠水液代谢和血液流变学的影响,探讨其作用机制。方法:将50只健康SD大鼠随机分为5组,每组10只,分别是正常组,天南星高,低剂量组(1.2,0.3 g·kg·d-1),虎掌南星高,低剂量组(1.2,0.3 g·kg·d-1)。以排尿指数、排便量、大便含水量、颌下腺病理组织形态、肾脏中水通道蛋白2(aquaporin 2,AQP2)含量、血清中环磷腺苷/环磷酸鸟苷(adenosine cyclic 3′,5′-hydrogen phosphate/current good manufacture practices,cAMP/cGMP)和三磷酸腺苷酶(ATPase,Na+-K+-ATP酶)含量为水液代谢评价指标,以全血黏度(whole blood viscosity,WBV)和红细胞聚集指数(erythrocyte aggregation index,RE)为血液流变学评价指标,分别来比较天南星与虎掌南星对大鼠水液代谢和血液流变学作用大小。结果:与正常组比较,高剂量组天南星和虎掌南星均使大鼠排便量、大便含水量和AQP2表达明显性降低(P<0.05),颌下腺组织中腺泡萎缩,排尿指数,cAMP/cGMP和Na+-K+-ATP酶含量明显升高(P<0.05),WBV和RE明显升高(P<0.05);但两组间差异无统计学意义。结论:高剂量的天南星和虎掌南星对大鼠水液代谢和血液流变学均有明显影响,表明临床用药时应注意天南星和虎掌安全性,为2种药物临床上的合理用药提供了理论依据。

Objective:To discuss the effect of Arisaema Rhizoma and Pinelliae Pedatisectae Rhizoma on water metabolism and hemorheolog in rats.Method:A total of 50 healthy SD rats were randomly divided into 5 groups,namely normal group,high-dose Arisaema Rhizoma group(1.2 g·kg·d-1),low-dose Arisaema Rhizoma group(1.2 g·kg·d-1),high-dose Pinelliae Pedatisectae Rhizoma group(1.2 g·kg·d-1)and low dose Pinelliae Pedatisectae Rhizoma group(0.3 g·kg·d-1),with 10 rats in each group.The effects of Arisaema Rhizoma and Pinelliae Pedatisectae Rhizoma on water metabolism and hemorheology were compared,with the micturition index,fecal volume,fecal water content,pathological morphology of submandibular gland,aquaporin(AQP2)content in kidney,adenosine cyclic 3′,5′-(hydrogen phosphate)/current good manufacture practices(cAMP/cGMP)and Na+-K+-ATP enzyme content in serum as the evaluation indexes of water metabolism,and the whole blood viscosity(WBV)and erythrocyte aggregation index(RE)as the evaluation indexes of hemorheolog.Result:Compared with normal group,the fecal volume,fecal water content and AQP2 expression decreased significantly,acinus of submandibular gland were atrophied,micturition index,cAMP/cGMP and Na+-K+-ATP enzyme content in serum were increased significantly,WBV and RE were increased significantly in high-dose Arisaema Rhizoma and Pinelliae Pedatisectae Rhizoma groups(P<0.05),but with no significant difference between them.Conclusion:High-dose Arisaema Rhizoma and Pinelliae Pedatisectae Rhizoma groups had significant effects on water metabolism and hemorheology in rats,suggesting that attention shall be given to the safety of Arisaema Rhizoma and Pinelliae Pedatisectae Rhizoma.And this study provides a theoretical basis for the rational use of the two traditional Chinese medicines in clinic.