一株木防己拟盘多毛孢的鉴定及其次生代谢产物分析

Identification of Pestalotiopsis cocculi and Its Secondary Metabolite Analysis

目的:研究分离自葡萄状枝瑚菌Ramariabotrytis子实体的16072314菌株分类学地位及其次生代谢产物成分。方法:采用形态学观察结合ITS序列分析法对16072314菌株进行菌种鉴定;利用硅胶柱色谱、凝胶柱色谱、制备薄层色谱和半制备高效液相色谱对16072314菌株固体发酵产物的化学成分进行分离纯化,根据理化性质及波谱数据分析鉴定化合物。结果:经鉴定16072314菌株为木防己拟盘多毛孢Pestalotiopsis cocculi;从16072314菌株的代谢产物中分离纯化了8个化合物,分别鉴定为7-羟基香豆素,阿魏酸,麦角甾醇,对羟基苯甲醛,对羟基苯乙醇,齐墩果酸,β-谷甾醇,过氧麦角甾醇。结论:8个化合物均为首次从木防己拟盘多毛孢中分离得到,阿魏酸为首次从拟盘多毛孢属中分离得到。本研究为拟盘多毛孢属的真菌资源利用和开发奠定了基础。

Objective:To identify the fungus strain 16072314 isolated from fruiting body of Ramariabotrytis and study its secondary metabolites.Method:The strain 16072314 was identified by morphological observation and ITS sequence analysis.Many technologies such as silica gel column chromatography,Sephadex LH-20 columns,preparative TLC and semipreparative high performance liquid chromatography(HPLC)were used to separate and purify the chemical constituents of solid medium from strain 16072314,and their structures were identified by physiochemical properties and spectral data.Result:The strain 16072314 belonged to Pestalotiopsis cocculi.Eight compounds were isolated from the solid medium of this strain and elucidated as 7-hydroxycoumarin,ferulic acid,ergosterol,p-hydroxybenzaldehyde,4-hydroxyphenethyl alcohol,oleanic acid,beta-sitosterol,ergosterol-5,8-peroxide.Conclusion:Eight compounds were first isolated from Pestalotiopsis cocculi,and ferulic acid 2 was first isolated from the Pestalotiopsis.The result would lay the foundation for utilization and development of Pestalotiopsis.