壳聚糖复方对高脂饲料致肥胖大鼠减肥作用的研究

Antiobesity Function of the Chitosan Compounds on Fatty Rats Established by High Fat Diet

为研究壳聚糖复方(壳聚糖、白芸豆提取物、葡萄籽提取物和富硒酵母)的减肥作用,除空白组外,其余大鼠喂养高脂饲料2周建立肥胖大鼠模型,建模成功后再随机分为模型组、奥利司他组(48 mg/kg)、壳聚糖配方高(0.812 g/kg)、中(0.406 g/kg)、低(0.203 g/kg)剂量组,连续给药6周后检测各组大鼠体重、Lee’s指数、肝体比、脂体比,血清甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、瘦素、脂联素、谷丙转氨酶(ALT)、谷草转氨酶(AST)及肝脏超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化酶(GSH-Px)等指标。结果显示与模型组相比,壳聚糖复方高剂量能显著降低肥胖大鼠的终末体重、肝体比、血清TG、TC含量及AST、ALT活性(P<0.05),极显著降低Lee’s指数、脂体比、血清LDL-C、瘦素及肝脏MDA含量(P<0.01),极显著提高血清脂联素含量及肝脏SOD活性(P<0.01),显著提高肝脏GSH-Px酶活力(P<0.05);壳聚糖复方中剂量能显著降低肥胖大鼠的终末体重、Lee’s指数、肝体比、脂体比、TG、TC、瘦素、MDA含量及血清ALT、AST活力(P<0.05),极显著降低LDL-C含量(P<0.01),显著升高血清脂联素含量及肝脏SOD活性(P<0.05),表明壳聚糖复方具有一定的减肥作用,其作用机制可能与调节机体脂肪代谢相关激素水平,加快机体的脂质代谢,减少氧化损伤等有关。

To study the antiobesity function of the chitosan compounds(chitosan,white kidney bean extracts,grape seed extract and selenium-enriched yeast),except the blank control group rats,the rest rats were feed with high fat diet for 2 weeks to establish fatty animal models,and then the successful model of the rats were randomly divided into model group,orlistat group(48 mg/kg),chitosan compounds high(0.812 g/kg),middle(0.406 g/kg)and low(0.203 g/kg)dose groups.After 6 weeks of administration,the changes of weight,Lee’s index,liver index,fat index,the contents of serum triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),leptin,adiponectin,liver malondialdehyde(MDA),and the activity of serum alanine aminotransferase(ALT),glutamic oxaloacetylase(AST),liver superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)were detected.Compared with the rats in model group,the high dose of chitosan compounds could significantly lower the weight,liver index,the contents of serum TG and TC,the activity of AST and ALT(P<0.05),remarkably lower the Lee’s index,fat index,the contents of the serum LDL-C,leptin,and the liver MDA(P<0.01),remarkably improve the contents of the serum adiponectin and the activity of liver SOD(P<0.01)and significantly improve the activity of liver GSH-Px(P<0.05).The middle dose of chitosan compounds(0.406 g/kg)could significantly lower the weight,Lee’s index,liver index,fat index,the contents of the serum TG,TC,leptin and liver MDA,the activity of serum ALT and AST(P<0.05),and remarkably lower the contents of LDL-C(P<0.01),and significantly improve the contents of adiponectin and the activity of liver SOD(P<0.05).Chitosan compounds had the antiobesity function,and its mechanism would be related to regulating the level of hormone related to fat metabolism,accelerating lipid metabolism and reducing oxidative damage.