基于益生菌调节的太平鸡回肠SNP位点分析

SNP Site Analysis of Taiping Chicken Ileum Based on Probiotic Regulation

为挖掘益生菌调节下太平鸡回肠SNP位点,将120只太平鸡随机分为对照组与益生菌组,采用高通量测序技术对太平鸡回肠转录组的SNP位点进行分析。结果显示:SNP类型中,转换类型明显高于颠换类型,对照组中转换类型占22.55%~27.45%,颠换类型占11.43%~13.87%;益生菌组与对照组结果相似,转换类型(22.53%~27.46%)明显高于颠换类型(11.37%~13.94%)。SNP位置分析表明,对照组和益生菌组总SNP位点分别为4684072和4647171个,其中位于内含子和基因间区的SNP位点最多。另外,在SNP功能分析中,同义突变SNP占比最高,在对照组和益生菌组中分别占7.01%和7.10%;非同义突变SNP在对照组和益生菌组中占比分别为2.97%和2.98%。该研究结果可为今后太平鸡的分子标记开发、遗传连锁图谱构建以及辅助育种等提供基础数据。

In order to excavate SNP sites in ileum of Taiping Chicken regulated by probiotics,120 Taiping chickens were randomly divided into control group and probiotic group.The SNP sites in the transcriptome of Taiping chicken’s ileum were analyzed by high throughput sequencing technique.The results showed that the transition proportion was significantly higher than that of the transversion.The transition of control group accounted for 22.55%-27.45%,and the transversion accounted for 11.43%-13.87%.The results of probiotic group were similar to those of control group,and the transition proportion(22.53%-27.46%)was significantly higher than that of the transversion(11.37%-13.94%).The analysis of SNP locations showed that the total number of SNP sites in the control group and the probiotic group was 4684072 and 4647171 respectively,among which the most SNP sites were located in introns and intergenic.In the analysis of SNP function,the proportion of synonymous mutations of SNP was the highest,which was 7.01%in the control group and 7.10%in the probiotic group.Nonsynonymous mutations of SNP accounted for 2.97%in the control group and 2.98%in the probiotic group.This study could provide basic data for the development of molecular markers,the construction of genetic linkage map and the assistant breeding of Taiping Chicken in the future.