从TGF-β1-PI3K/Akt信号通路研究妇科千金胶囊治疗宫腔粘连大鼠作用机制

Mechanism of Fuke Qianjin Capsules in treating intrauterine adhesion in rats through TGF-β1-PI3K/Akt signaling pathway

从TGF-β1-PI3K/Akt信号通路探讨妇科千金胶囊对宫腔粘连(intrauterine adhesion,IUA)大鼠抗子宫内膜纤维化的作用。以雌性SD大鼠为对象,采用机械损伤+感染建立IUA大鼠模型,随机分为正常组、假手术组、戊酸雌二醇组(0.63 mg·kg^-1·d^-1,B)及妇科千金胶囊高(1.008 g·kg^-1·d^-1,QL-H)、中(0.504 g·kg^-1·d^-1,QJ-M)、低(0.252 g·kg^-1·d^-1,QJ-L)剂量组。药物灌胃21 d后处死大鼠,腹主动脉采血后留取子宫及肝脏,肉眼观察子宫大体形态;HE染色法观察子宫组织及肝组织病理形态学变化;Masson染色法观察子宫组织纤维化程度;ELISA法检测血清中TGF-β1,TNF-α,IL-6表达量;Western blot法检测子宫组织TGF-β1,PI3K,Akt,p-Akt蛋白的表达。结果显示,妇科千金胶囊可改善IUA大鼠子宫组织病理变化,对肝组织无损伤,可显著降低血清中TGF-β1,TNF-α,IL-6表达量(P<0.01);显著降低子宫组织中TGF-β1,PI3K,p-Akt蛋白表达(P<0.05,P<0.01)。表明妇科千金胶囊可通过调节TGF-β1-PI3K/Akt信号通路发挥抗子宫内膜纤维化功效,从而达到治疗IUA大鼠的作用,尤以妇科千金胶囊中剂量组效果最佳。

To explore the effect of Fuke Qianjin Capsules on anti-endometrial fibrosis in intrauterine adhesion(IUA)rats through TGF-β1-PI3 K/Akt signaling pathway.With female SD rats as the object,IUA rat models were established through mechanical injury and infection,and they were randomly divided into normal group,sham operation group,Bujiale group(0.63 mg·kg^-1·d^-1),and high-dose Fuke Qianjin Capsules group(1.008 g·kg^-1·d^-1),medium-dose Fuke Qianjin Capsules group(0.504 g·kg^-1·d^-1),low-dose Fuke Qianjin Capsules group(0.252 g·kg^-1·d^-1).The rats were sacrificed 21 days after drug administration,and the uterus and liver were removed after blood collection from the abdominal aorta.The morphology of the uterus was observed with the naked eyes;the pathological and morphological changes of the uterine tissue and liver were observed by HE staining;the degree of fibrosis of the uterine tissue was observed by Masson staining;the expressions of TGF-β1,TNF-αand IL-6 in serum were detected;the expressions of TGF-β1,PI3 K,Akt,p-Akt protein in uterine tissue were detected by Western blot.The results showed that Fuke Qianjin Capsules could improve the pathological changes of uterine tissues in IUA rats,without damage to liver tissues,and reduce the expressions of TGF-β1,TNF-αand IL-6 in serum(P<0.01);significantly reduce TGF-β1,PI3 K,p-Akt protein expression in uterine tissues(P<0.05,P<0.01).It is indicated that Fuke Qianjin Capsules could exert the anti-endometrial fibrosis effect by regulating the TGF-β1-PI3 K/Akt signal pathway,so as to achieve the effect in treating IUA rats,especially with the best effect in medium-dose Fuke Qianjin Capsules group.