摘要
Background:Circular RNA ciRS-7 has been reported to be involved in the progression of various cancers.However,ciRS-7 expression and its role in clear cell renal cell carcinoma(ccRCC)progression remains unclear.This study aimed to investigate the effect of ciRS-7 expression on ccRCC and the related signaling pathway.Methods:ciRS-7 expression was analyzed using quantitative reverse transcription polymerase chain reaction in 87 pairs of ccRCC and matched adjacent normal tissues.The role of ciRS-7 in ccRCC cell proliferation and invasion was determined using the cell counting kit-8 and invasion assays,respectively.Potential mechanisms underlying the role of ciRS-7 in promoting ccRCC progression were explored by Western blotting.The relationship between the expression of ciRS-7 and features of ccRCC was analyzed by the Chi-square test and progression-free survival was determined using a Kaplan-Meier plot.Results:ciRS-7 was overexpressed in ccRCC tissues compared with that in matched adjacent normal tissues.In addition,ciRS-7 up-regulation was closely associated with tumor diameter(P=0.050),clinical stage(P=0.009),and distant metastasis(P=0.007).ciRS-7 knockdown in 786O and 769P cells markedly inhibited their proliferative and invasive abilities.In addition,ciRS-7 inhibition reduced phosphorylated epidermal growth factor receptor(p-EGFR)and phosphorylated serine/threonine kinase(p-Akt)levels.Conclusions:ciRS-7 up-regulation could promote ccRCC cell proliferation and invasion,which may be related with the EGFR/Akt signaling pathway.ciRS-7 might be a potential ccRCC therapeutic target.
