结肠癌组织中SDC1蛋白的表达变化及意义

Expression changes and significance of SDC1 protein in colon cancer tissues

目的检测结肠癌组织中多配体蛋白聚糖1(SDC1)蛋白的表达,分析SDC1蛋白表达与患者临床病理特征及上皮间质转化标志物上皮型钙黏蛋白(E-cadherin)、神经型钙黏蛋白(N-cadherin)和预后的关系。方法收集130例结肠癌患者癌组织和癌旁正常组织的石蜡样本,采用免疫组化法检测组织样本中SDC1、E-cadherin、N-cadherin蛋白表达;分析癌组织中SDC1蛋白表达与患者临床病理特征的关系;Kaplan-Meier生存模型分析SDC1蛋白表达对患者预后的影响;Spearman等级相关分析SDC1蛋白表达与E-cadherin、N-cadherin蛋白表达的相关性。结果结肠癌组织与癌旁正常组织中SDC1蛋白阳性表达率分别为22.31%(29/130)、91.54%(119/130),二者比较差异有统计学意义(P<0.05)。结肠癌组织中SDC1蛋白表达与肿瘤分化程度、浸润深度、淋巴结转移、远处转移和TNM分期有关(P均<0.05)。Log-rank检验结果显示,SDC1蛋白阴性表达者术后5年总生存率低于SDC1蛋白阳性表达者(P<0.05)。Spearman相关检验结果显示,结肠癌组织中,SDC1蛋白表达与E-cadherin蛋白表达呈正相关(rs=0.753,P=0.016),SDC1蛋白表达与N-cadherin蛋白表达呈负相关(rs=-0.682,P=0.028)。结论结肠癌组织中SDC1蛋白呈低表达,且与患者病情恶性进展、预后不良有关;SDC1蛋白表达下降可降低E-cadherin蛋白表达、增加N-cadherin蛋白表达,诱发上皮间充质转化过程,进而促进侵袭和转移。

Objective To detect the expression of polyligand proteoglycan 1(SDC1)protein in the colon cancer tissues,to analyze the relationship between SDC1 protein expression and clinicopathological characteristics,epithelial-mesenchymal transition markers E-cadherin,N-cadherin,and the prognosis of patients.Methods The paraffin samples of cancer tissues and adjacent normal tissues from 130 patients with colon cancer were collected.Immunohistochemistry was used to detect the expression levels of SDC1,E-cadherin,and N-cadherin proteins in the tissue samples.The relationship between expression of SDC1 protein in the cancer tissues and the clinical pathological characteristics of the patients were analyzed.Kaplan Meier survival model was used to analyze the influence of SDC1 protein expression on the prognosis of patients.Spearman rank correlation was used to analyze the correlation between SDC1 protein expression and E-cadherin,N-cadherin protein expression.Results The positive rate of SDC1 protein expression in the colon cancer tissues was 22.31%(29/130),which was lower than that of the adjacent tissues[91.54%(119/130)],with statistically significant difference(P<0.05).In the colon cancer tissues,the expression of SDC1 protein was related to the degree of tumor differentiation,depth of invasion,lymph node metastasis,distant metastasis and TNM staging(all P<0.05).Log Rank test results showed that the 5-year overall survival rate of patients with negative expression of SDC1 protein was lower than that of patients with positive expression of SDC1 protein(P<0.05).Spearman correlation test results showed that in the colon cancer tissues,the expression of SDC1 protein was positively correlated with the expression of E-cadherin protein(rs=0.753,P=0.016),and the expression of SDC1 protein was negatively correlated with the expression of N-cadherin protein(rs=-0.682,P=0.028).Conclusions The expression of SDC1 protein in the colon cancer tissues is low,and it is related to the malignant progression and poor prognosis of patients.Decreased expression of SDC1 protein can reduce the expression of E-cadherin protein,increase the expression of N-cadherin protein,and induce epithelial-mesenchymal transition,and then promote invasion and metastasis.