CpG ODN B对鸡新城疫重组杆状病毒疫苗的免疫增强效应

Immune Enhancement of CpG ODN B on Recombinant Baculovirus Vaccine Against Newcastle Disease in Chickens

目的:探究CpG ODN作为疫苗佐剂对鸡新城疫重组杆状病毒疫苗的免疫效果。方法:将CpG ODN B/P这2种类型的免疫佐剂分别与鸡新城疫重组杆状病毒载体疫苗BV-BXY-F混合后,以鼻腔免疫方式进行鸡体免疫,并进行攻毒保护实验,通过对鸡体的临床症状观察,以及检测免疫保护率、中和抗体水平、淋巴细胞增殖活性、细胞因子含量,分析各CpG ODN对鸡新城疫疫苗免疫效果的影响。结果:BV-BXY-F+CpG ODN B免疫组鸡在免疫后42 d中和抗体效价最高,免疫保护率100%,中和抗体效价,sIgA、IgG、IgA、IFN-γ、IL-2、IL-4、CD4^+T细胞、CD8^+T细胞含量较BV-BXY-F+CpG ODN P组分别提高了32.08%、8.44%、13.56%、23.71%、50.20%、132.97%、39.76%、38.43%、25.99%,且差异显著(P<0.05),与单独免疫组BV-BXY-F相比分别提高了13.71%、89.39%、10.41%、21.26%、159.17%、52.71%、237.84%、80.51%、75.57%。结论:CpG ODN B对增强鸡新城疫重组杆状病毒疫苗的免疫增强效果最好。

Objective:To explore the immune enhancement of CpG ODN B as a vaccine adjuvant on recombinant baculovirus vaccine against Newcastle disease in chickens.Methods:Two types of CpG ODN B/P were used as immune adjuvants and the recombinant baculovirus vector vaccine BV-BXY-F was mixed,and the nasal immunization method and the challenge protection experiment were carried out in chickens.The immune improvement effect CpG ODN in the recombinant baculovirus vector vaccine BV-BXY-F was evaluated by detection of clinical symptoms,immune protection rate,the neutralizing antibody level,protection efficacy,lymphocyte proliferation activity,and cytokines in chickens.Analyze the impact of each CpG ODN on the immune effect of chicken Newcastle disease vaccine.Results:The neutralizing antibody titer of chickens with BV-BXY-F+CpG ODN B combined immunization group was the highest at 42 days after immunization,and the immune protection rate of chickens with BV-BXY-F+CpG ODN B was 100%.sIgA antibody content,neutralizing antibody titer,IgG,IgA,IFN-γ,IL-2,IL-4,CD4^+T cells,CD8^+T cells compared with BV-BXY-F+CpG ODN P,increased by 8.44%,32.08%,13.56%,23.71%,50.20%,132.97%,39.76%,38.43%,25.99% respectively,and the difference was significant(P<0.05).Compared with the independent immunization group of chickens with BV-BXY-F increased by 13.71%,89.39%,10.41%,21.26%,159.17%,52.71%,237.84%,80.51%,75.57%.Conclusion:BV-BXY-F+CpG ODN B is the best immune group,and CpG ODN B is the best type of immune adjuvant.