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反向PCR法构建TRAF6蛋白截短体质粒 被引量：1

Construction of Plasmid for TRAF6 Truncated Protein by Inverse PCR
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摘要 目的:构建肿瘤坏死因子受体相关因子6(TRAF6)截短体质粒pCMV-Myc-TRAF6 N和pCMV-Myc-TRAF6 C。方法:采用反向PCR法。结果:经双酶切鉴定,分别得到约900和750 bp的TRAF6截短体质粒pCMVMyc-TRAF6 N和pCMV-Myc-TRAF6 C,符合预期;转染293FT细胞后,Western印迹检测到TRAF6 N和TRAF6 C的表达。结论:构建了TRAF6蛋白截短体质粒,有助于进一步验证TRAF6的生物学功能。 Objective:To construct pCMV-Myc-TRAF6 N and pCMV-Myc-TRAF6 C plasmids.Methods:The inverse PCR method was used.Results:The plasmid obtained by inverse PCR was confirmed by double enzymatic digestion,which was consistent with the expected size.The expression of TRAF6 N and TRAF6 C after transfection of 293 FT cells was detected by Western blotting.Conclusion:pCMV-Myc-TRAF6 N and pCMV-MycTRAF6 C were successfully constructed,which is helpful to further verify the biological function of TRAF6.
作者 周晨辰 陆琤 何园 查玉华 张硌 ZHOU Chen-Chen;LU Cheng;HE Yuan;ZHA Yu-Hua;ZHANG Luo(Medical Engineering Department,Southern District of the Fifth Medical Center of PLA General Hospital,Beijing 100071,China)
机构地区 解放军总医院第五医学中心南院区医学工程科
出处 《生物技术通讯》 CAS 2020年第4期448-450,454,共4页 Letters in Biotechnology
基金 北京市自然科学基金(5192021)。
关键词 肿瘤坏死因子受体相关因子6(TRAF6) TRAF6 N TRAF6 C 反向PCR法 tumor necrosis factor receptor associated factor 6(TRAF6) TRAF6 N TRAF6 C inverse PCR method
分类号 Q78 [生物学—分子生物学]
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生物技术通讯
2020年 第4期

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