黄秋葵对南方根结线虫抗性的转录组分析

Transcriptome Analysis of Okra Resistance for Meloidogyne incognita

为了解黄秋葵抗南方根结线虫相关基因组学,利用Illumina Hi-seq TM2500高通量测序技术研究受南方根结线虫侵染后黄秋葵种质12C2转录组基因的差异表达。结果表明,接种南方根结线虫18 h后,从接种和未接种的黄秋葵种质12C2根尖中共获得71.49 Gb有效数据,Q30碱基百分比均达到94.0%以上。共获得2318个差异表达基因(DEGs),包括1156个上调基因,1162个下调基因,其中功能注释基因2202个。根据unigene库序列进行GO、KOG和KEGG注释,细胞壁代谢相关基因——内切葡聚糖酶基因家族、聚半乳糖醛酸酶基因家族、葡聚糖内-1,3-β葡糖苷酶基因家族和果胶裂解酶基因家族下调表达,植物激素代谢相关基因——生长素响应蛋白基因、生长素流入运输载体基因下调表达,茉莉酸合成酶基因上调表达,调控相关基因表达的WRKY和MYB转录因子基因家族上调表达,参与植物细胞的膜联蛋白基因家族上调表达,植物细胞周期蛋白基因家族下调表达。本研究结果为开展黄秋葵抗南方根结线虫基因组学和分子生物学研究奠定了基础。

In order to understand the related genomics of okra germplasm 12 C2 resistance for Meloidogyne incognita,the differential expression genes(DEGs)was studied using Illumina Hiseq TM2500 high-throughput sequencing technology.The results showed that there were 71.49 Gb glean data from the root tip treated and untreated with M.incognita after 18 h,the base percentage of Q30 was more than 94.0%.Among them,there were 2318 DEGs,including 1156 up-regulated genes and 1162 down-regulated genes,among which 2202 genes were annotated.The GO,KOG and KEGG are annotated according to the sequence of unigene library.The cell wall metabolism-related genes,including endoglucanase gene family,polygalacturonase gene family,glucan endo-1,3-beta-glucosidase gene family and pectate lyase gene family were down-regulated.Among the plant hormone metabolism-related genes,auxin responsive protein gene and auxin influx carrier gene were down-regulated,and jasmonic acid synthase gene was up-regulated.WRKY and MYB gene family that regulate the expression of related genes were down-regulated.Annexin gene family and cyclin gene family involved in the expression of plant cells related genes were up and down-regulated,respectively.The results would provide a foundation for studying on genomics and molecular biology of okra resistance for M.incognita.