摘要
为揭示贵州省市售婴儿配方乳粉(PIF)中克罗诺杆菌的污染情况及种群特征,本研究以350份PIF样品为研究对象,对PIF中的克罗诺杆菌进行分离鉴定;利用API 20E生化鉴定法、16S rRNA测序法和多位点序列分型(MLST)法对分离株进行生化表型分型和基因分型,最后利用Kirby-Bauer纸片扩散法进行菌株的耐药性分析。结果表明,350份样品中共分离出21株克罗诺杆菌,污染率为6%。21株克罗诺杆菌被分为6个生化表型,3个类群和11个序列型,其中ST193和ST157为分离自贵州省市售PIF的克罗诺杆菌的优势序列型。耐药性分析结果表明,21株克罗诺杆菌对四环素和万古霉素具有高度的耐药性,而对氨苄西林、庆大霉素和氯霉素较为敏感。本研究结果为贵州省市售PIF中克罗诺杆菌的防控提供了理论依据。
The aim of this study was to indicate the population and characteristics of Cronobacter spp. in powdered infant formula(PIF) collected in Guizhou province. A total of 350 PIF samples were used as research objectes to isolate and identify the Cronobacter strains. The biochemical phenotypes and genotypes of isolates were performed by API 20 E system, 16 S rRNAsequencing and multilocus sequence typing(MLST) methods. Finally, the antibiotic susceptibility of isolates was evaluated using Kirby-Bauer diffusion method. The results indicated that 21 Cronobacter strains were isolated from 350 PIF samples, with a contamination rate of 6%. These isolates were divided into 6 biochemical phenotypes, 3 groups and 11 sequence types(STs). Among them, ST193 and ST157 were the dominant STs of Cronobacter isolated from commercially available PIF in Guizhou. The results of antibiotic susceptibility showed that 21 isolates were highly resistant to tetracycline and vancomycin, while, sensitive to smpicillin, gentamicin, and chloramphenicol. Our findings provided a theoretical basis for the prevention and control of Cronobacter in commercially available PIF collected from Guizhou.
作者
陈曦
黄道梅
孟繁博
郑秀艳
李国林
CHEN Xi;HUANG Daomei;MENG Fanbo;ZHENG Xiuyan;LI Guolin(Institute of Integrated Agricultural Development Research,Guizhou Academy of Agriculrural Sciences,Guiyang,Guizhou 550006)
出处
《核农学报》
CAS
CSCD
北大核心
2020年第10期2290-2298,共9页
Journal of Nuclear Agricultural Sciences
基金
贵州省特色农产品辐照保鲜技术服务企业行动计划(黔科合平台人才[2016]5712)
贵州省特色农产品辐照保鲜与加工工程技术研究中心(2016GZ67305)。
关键词
婴儿配方乳粉
克罗诺杆菌
分离鉴定
分型
耐药性
powdered infant formula
Cronobacter
isolation and identification
typing
antibiotic susceptibility