摘要
目的:建立HPLC-ELSD法测定康寿丸中人参皂苷Rg1、Re、Rb1的含量。方法:采用Waters CORTECS T3 C18色谱柱(150 mm×4.6 mm,2.7μm),以乙腈-水梯度洗脱,流速0.8 mL/min,柱温30℃;采用蒸发光散射检测器检测,漂移管温度50℃,载气流速1.6 L/min。结果:人参皂苷Rg1、Re、Rb1分别在进样量为0.0795~3.1792μg、0.0770~3.0772μg、0.0953~3.8112μg范围内呈良好的线性关系,相关系数分别为0.9989,0.9993,0.9994。三种人参皂苷的平均回收率分别为99.8%(RSD=1.0%)、100.9%(RSD=1.7%)、99.8%(RSD=1.3%)(n=6)。结论:本方法灵敏度高,简便,重复性好,可用于康寿丸中人参皂苷的含量测定。
Objactive:To establish an HPLC-ELSD method for simultaneous determination of ginsenoside Rg1,Re,and Rb1 in Kangshou pills.Methods:The analysis was performed on Waters CORTECS T3 C18 column(4.6 mm×150 mm,2.7μm)with the mobile phase comprising of acetonitrile-water,flowing at 0.8 mL/min in a gradient elution manner and on 30℃thermostatic.The drift tube temperature was 50℃and the gas flow was 1.6 L/min.Results:Ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 were linear within the ranges of 0.0795~3.1792μg(r=0.9989)、0.0770~3.0772μg(r=0.9993)、0.0953~3.8112μg(r=0.9994),respectively.The average recovery were 99.8%,100.9%and 99.8%,and the RSD were 1.0%,1.7%and 1.3%(n=6)respectively.Conclusion:The method is accurate,convenient and reproducible and can be used for the quality control of Kangshou pills.
作者
黄天山
杨洁瑜
顾利红
HUANG Tianshan;YANG Jieyu;GU Lihong(Guangzhou Institute of Drug Control,NMPA Key Laboratory of Quality Control of Chinese Patent Medicine,Guangzhou 510160,China)
出处
《药品评价》
CAS
2020年第15期27-30,共4页
Drug Evaluation
基金
2019年度药品标准提高品种(2019Z048)。
