江苏涟水地区健康孕妇红细胞相关参数参考区间的建立及验证

Establishment and validation of reference intervals for erythrocyte parameters of healthy pregnant women in Lianshui area of Jiangsu

目的建立适合该科室健康孕妇红细胞相关参数的生物参考区间并验证其实用性。方法根据孕周的不同,将391例健康孕妇分为早孕组(122例)、中孕组(100例)和晚孕组(169例)。另选择与健康孕妇年龄相近的248例健康非孕妇作为对照组。检测所有研究对象红细胞计数(RBC)、血红蛋白(Hb)、红细胞比容(HCT)、红细胞平均血红蛋白含量(MCH)、红细胞平均血红蛋白浓度(MCHC)、红细胞平均体积(MCV)、红细胞分布宽度变异系数(RDW-CV)、红细胞分布宽度标准差(RDW-SD),建立可用于诊断孕期红细胞相关参数的参考区间并进行验证。结果不同孕期组与对照组RBC、Hb、HCT、MCV、MCHC水平比较,差异均有统计学意义(P<0.05);早孕组和中孕组与晚孕组比较,RDW-CV、RDW-SD水平差异均有统计学意义(P<0.05);孕妇组与对照组MCH水平比较,差异无统计学意义(P>0.05);建立的实验室孕早、中、晚期红细胞相关参数的参考区间经验证符合要求。结论孕早、中、晚期孕妇红细胞相关参数的参考区间与非孕妇有明显差异,各实验室应建立孕期红细胞相关参数的参考区间,对临床评估孕妇的健康水平,促进优生优育有非常重要的作用。

Objective To establish a bio-reference interval suitable for the parameters of red blood cells of healthy pregnant women in the laboratory and to verify its applicability.Methods According to the different gestational weeks,391 healthy pregnant women were divided into early pregnancy group(122 cases),mid-pregnancy group(100 cases)and late pregnancy group(169 cases).248 healthy non-pregnant women of similar age as pregnant women were selected as control group.Red blood cell count(RBC),hemoglobin(HB),hematocrit(HCT),mean corpuscular hemoglobin content(MCH),mean corpuscular hemoglobin concentration(MCHC),mean corpuscular volume(MCV),red blood cell distribution width variation coefficient(RDW-CV)and standard deviation of red blood cell distribution width(RDW-SD)were measured to establish a reference for the diagnosis of red blood cell related parameters during pregnancy,and the interval was verified.Results There were significant differences in RBC,Hb,HCT,MCV and MCHC among the four groups(P<0.05).RDW-CV and RDW-SD had significant differences between the early and middle pregnancy groups and the late pregnancy groups(P<0.05).There was no significant difference in MCH among the four groups(P>0.05).The reference interval for establishing the parameters of erythrocyte in the early,middle and late pregnancy in the laboratory was proved to be in line with the requirements.Conclusion There are significant differences in reference intervals of erythrocyte-related parameters between early,middle and late pregnant women and non-pregnant women.The reference intervals of erythrocyte-related parameters during pregnancy should be established in laboratories.This is very important for clinical evaluation of the health level of pregnant women and promotion of eugenic and eugenic birth.