长链非编码RNA HIF1A-AS2在结直肠癌组织中的表达及临床意义

Expression and clinical significance of Lnc RNA HIF1A-AS2 in colorectal cancer tissues

目的检测LncRNA HIF1A-AS2在结直肠癌组织中的表达,并探讨其临床意义。方法选取2012年12月至2015年12月行结直肠癌患者根治术的114例结直肠癌患者临床病理资料;收集其癌组织及相对应的癌旁组织(距病灶2 cm)和正常组织(距癌灶边缘>5 cm)标本。通过qRT-PCR检测结直肠癌组织、癌旁组织及癌旁正常组织中HIF1A-AS2相对表达水平。取114例入组患者HIF1A-AS2表达水平的平均数,将HIF1A-AS2表达水平大于等于平均数的89例纳入高表达组,其余纳入低表达组;分析HIF1A-AS2表达与临床病理特征之间的关系;Kaplan-Meier生存曲线分析两组患者术后5年生存情况,Log-rank检验比较生存差异;COX比例风险回归模型分析预后不良的独立危险因素;P<0.05为差异有统计学意义。结果结直肠癌组织中HIF1A-AS2相对表达量高于癌旁组织及正常组织(P<0.001)。HIF1A-AS2表达与临床分期及组织分化程度显著相关(P<0.05)。HIF1A-AS2高表达组患者术后5年总生存率低于HIF1A-AS2低表达组(Log-rankχ^2=5.037,P=0.025)。COX多因素分析显示淋巴结转移、临床分期Ⅳ期、中-低分化、有远处转移及HIF1A-AS2显著高表达是影响结直肠癌患者预后不良的独立危险因素(P<0.05)。结论结直肠癌组织中HIF1A-AS2显著高表达,与临床病理及预后密切相关,是影响结直肠癌患者预后不良的危险因素之一。

Objective To investigate the expression of LncRNA HIF1A-AS2 in colorectal cancer tissues and to explore its clinical significance.Methods Clinicopathological data of 114 patients,who were with colorectal cancer from December 2012 to December 2015 and underwent radical resection,were collected,including samples of the cancer tissue and corresponding adjacent tissues(2 cm away from the lesion)and adjacent normal tissues(5 cm away from the edge of the cancer lesion).The relative expression levels of HIF1A-AS2 in colorectal cancer tissues,adjacent tissues and adjacent normal tissues were detected by qRT-PCR.The average expression level of HIF1A-AS2 in 114 enrolled patients was evaluated,and 89 patients with HIF1A-AS2 expression level greater than or equal to the average were divided into the high-expression group,while the rest were divided into the low-expression group.The relationship between HIF1A-AS2 expression and clinicopathological features was analyzed.Kaplan-meier survival curve was usedto analyze the 5-years survival of patients in two groups by using log-rank test.COX proportional risk regression model were used to analyze the independent risk factors for prognosis.A P value of<0.05 was considered as statistically significant difference.Results The relative expression of HIF1A-AS2 in colorectal cancer tissues was higher than that in adjacent tissues and normal tissues(P<0.001).HIF1A-AS2 expression was significantly correlated with clinical stage and degree of differentiation in cancer tissue(P<0.05).The 5-year overall survival rate of the HIF1A-AS2 high-expression group was lower than that of the HIF1A-AS2 low-expression group=0.025).COX multi-factor analysis showed that lymph node metastasis,clinical stageⅣ,in poorly differentiated,with distant metastasis and HIF1A AS2 significantly high expression were the independent risk factors influencing the prognosis of patients with colorectal cancer(P<0.05).Conclusion HIF1A-AS2 is highly expressed in colorectal cancer tissues,which is closely related to clinicopathology and prognosis,and is one of the risk factors for poor prognosis of patients with colorectal cancer.