珍珠舌草胶囊全方对胃癌细胞增殖与凋亡的影响及机制研究

Effect and Mechanism of Zhenzhushecao Capsule on Proliferation and Apoptosis of Gastric Cancer Cells

目的珍珠舌草胶囊是对慢性萎缩性胃炎癌前病变具有良好临床疗效的中药复方制剂。通过珍珠舌草胶囊干预胃癌AGS细胞,观察其对细胞增殖和凋亡的影响,并对可能的作用机制进行初步探究,从而证实珍珠舌草胶囊的抗肿瘤活性,为临床更好地应用珍珠舌草胶囊提供理论和实验依据。方法应用MTT法检测珍珠舌草胶囊对胃癌AGS细胞生长的影响;应用流式细胞仪检测药物对胃癌AGS细胞周期和凋亡的影响;比色法测定肿瘤细胞中Caspase-3活性水平;药物干预期间观察细胞数量及形态变化。结果珍珠舌草胶囊全方能显著抑制胃癌细胞AGS的生长,并呈剂量和时间依赖性;阻滞细胞周期于G2/M期,明显促进胃癌细胞凋亡,上调Caspase-3的活性水平。结论珍珠舌草胶囊全方能够抑制AGS细胞增殖,阻滞细胞周期于G2/M期,促进细胞凋亡,促凋亡作用可能通过提高Caspase-3的活性实现。

Objective Zhenzhushecao Capsule(ZZSCC),a complex prescription with clinical effect on chronic atrophic gastritis.The present study investigated the effect and mechanism of Zhenzhushecao Capsule on proliferation and apoptosis of gastric cancer cell line AGS.Methods Cell viability was measured by MTT analysis.Cell cycle and apoptosis were detected by flow cytometry(FCM).Caspase-3 activity was measured by colorimetric method.The changes of cell morphologic appearance after treatment were observed.Results ZZSCC inhibited AGS cell proliferation in a dose-dependent manner,and blocked cell cycle progression at G2/M stage,and also induced cell apoptosis.ZZSCC significantly increased Caspase-3 activity after administration for 24,48 h.Conclusion The whole prescription of ZZSCC could inhibit AGS cell growth,induce cell cycle arrest and apoptosis in an dose-independent manner.The apoptosis promotion effect may be related to increased Caspase-3 activity.