傣痛消乙醇提取物对高尿酸血症小鼠及痛风性关节炎大鼠的影响

Effects of Daitongxiao Ethanol Extract on Hyperuricemia Mice and Gouty Arthritis Rats

目的观察傣痛消乙醇提取物对高尿酸血症小鼠及痛风性关节炎大鼠的影响。方法选取60只昆明种小鼠随机分为正常对照组、模型组、苯溴马隆组和傣痛消乙醇提取物低、中、高剂量组。模型组及各药物组小鼠均使用30 g/kg酵母进行灌胃造模,正常对照组小鼠给予等容量的蒸馏水;傣痛消乙醇提取物低、中、高剂量组在造模第4天分别以2.6、7.8、23.4 g/kg的傣痛消乙醇提取物灌胃,正常对照组和模型组给予等体积蒸馏水;苯溴马隆组在造模第6天以0.02 g/kg苯溴马隆灌胃,每日1次,连续4 d,末次给药1 h后摘眼球取血。选取60只Wistar大鼠随机分为正常对照组、模型组、秋水仙碱组、傣痛消乙醇提取物低、中、高剂量组。在造模前5 d傣痛消乙醇提取物各组分别以1.8、5.4、16.2 g/kg的傣痛消乙醇提取物灌胃;秋水仙碱组在造模前3 d以0.28 mg/kg秋水仙碱灌胃;正常对照组和模型组给予等体积蒸馏水,每日1次。在末次给药1 h后,模型组及药物组复制急性痛风关节炎模型,造模5 h后麻醉大鼠,腹主动脉取血。分别使用试剂盒检测大鼠和小鼠血清中尿酸(UA)、黄嘌呤氧化酶(XOD)、肌酐(CRE)、尿素氮(BUN)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和白细胞介素1β(IL-1β)的水平;观察大鼠造模前后关节肿胀情况。结果与正常对照组比较,模型组大鼠和小鼠UA、XOD、CRE、BUN、IL-6、TNF-α和IL-1β均显著升高(P<0.05),急性痛风性关节炎大鼠出现躁动不安、右足抬高、局部红肿、肤温变高和关节肿胀指数显著增加的情况。与模型组比较,傣痛消乙醇提取物低剂量组对高尿酸血症小鼠CRE和急性痛风性关节炎大鼠UA、CRE、IL-6、TNF-α改善不明显,其余各药物组中UA、XOD、CRE、BUN、IL-6、TNF-α、IL-1β水平均显著降低(P<0.05);药物组急性痛风性关节炎大鼠局部关节肿胀情况较模型组改善明显。结论傣痛消乙醇提取物对高尿酸血症小鼠和急性痛风性关节炎大鼠具有降尿酸、改善肾功能和抗炎作用。

Objective To observe the effects of Daitongxiao ethanol extract(DTXEE)on hyperuricemia mice and gouty arthritis rats.Methods 60 mice were randomly divided into normal control group,model group,Benbromarone group and low,medium and high dose groups of DTXEE.The model group and drug groups were given 30 g/kg yeast by gavage,while the normal control mice were given distilled water.On the 4th day after modeling,the low,medium and high DTXEE groups were given 2.6,7.8,23.4 g/kg DTXEE,respectively,and the model group and normal control group were given distilled water.The Benbromarone group was given 0.02 g/kg benbromarone on the 6th day,administration lasted for 4 d.The blood was taken from the eyeball 1 h after the last administration.60 Wistar rats were randomly divided into normal control group,model group,colchicine group,and low,medium and high dose groups of DTXEE.The DTXEE groups were gavaged with 1.8,5.4,16.2 g/kg of DTXEE respectively for 5 d before modeling,the colchicine group was gavaged with 0.28 mg/kg colchicine for 3 d before modeling,the normal control group and model group were given distilled water once a day.The acute gouty arthritis model was made 1 h later after the last administration in model and drug groups,5 h after modeling,rats were anesthetized and blood was taken from abdominal aorta.The levels of UA,XOD,CRE,BUN,IL-6,TNF-αand IL-1βin the serum of rats and mice were detected by the kits,and the joint swelling of rats before and after modeling were observed.Results Compared with the normal control group,UA,XOD,CRE,BUN,IL-6,TNF-α,IL-1βin the model group were significantly higher(P<0.05).Restlessness,elevation of right foot,local redness and swelling,increased skin temperature and joint swelling index were observed in acute gouty arthritis rats.Compared with model group,the improvement of CRE in hyperuricemia and UA,CRE,IL-6,TNF-αin acute gouty arthritis were not significant after the intervention of low-dose DTXEE.While the levels of UA,XOD,CRE,BUN,IL-6,TNF-α,IL-1βlevels were significantly lower in other drug groups(P<0.05).Moreover,the local joint swelling of rats with acute gouty arthritis in drug groups were improved better than model group.Conclusion DTXEE could reduce uric acid,improve renal function and anti-inflammatory in hyperuricemia mice and acute gouty arthritis rats.