香叶木素固体脂质纳米粒的制备及质量评价

Preparation and quality evaluation of diosmetin solid lipid nanoparticles

目的制备香叶木素固体脂质纳米粒并对其进行质量评价。方法采用溶剂注入法制备香叶木素固体脂质纳米粒,用Box-Benhnken效应面法优化处方,并通过包封率、微观形态、粒径分布和Zeta电位对香叶木素固体脂质纳米粒的质量进行评价。结果香叶木素固体脂质纳米粒最优处方组成:表面活性剂浓度3.39%,棕榈酸浓度0.116%,脂药质比为21:100,制备的香叶木素固体脂质纳米粒外观澄清透明,带淡蓝色乳光;平均粒径为(91.73±3.18)nm(n=3),PDI为0.228,电位为(-11.46±0.74)mV(n=3);包封率为95.13%,载药量为9.04%;透射电镜照片显示纳米粒大小均一,呈球形或类球形。结论该处方可用于香叶木素固体脂质纳米粒的制备,工艺简单,稳定可行。

Objective Preparation of diosmetin solid lipid nanoparticles and quality evaluation.Methods Diosmetin solid lipid nanoparticles were prepared by the solvent injection method.The formulation was optimized by the Box-Benhnken effect surface method.The quality of diosmetin solid lipid nanoparticles was evaluated by encapsulation efficiency,microscopic morphology,particle size distribution,and zeta potential.Results The optimal formulation composition of diosmetin solid lipid nanoparticles was as follows:surfactant concentration of 3.39%,palmitic acid addition amount of 29.10mg,lipid drug ratio:21:100.The prepared diosmetin solid lipid nanoparticles were clear and transparent,with light blue opalescence.The average particle size was(91.73±3.18)nm(n=3),PDI was 0.228,and the potential was(-11.46±0.74)mV(n=3).The encapsulation efficiency was 95.13%,and the drug loading was 9.04%.Transmission electron micrographs showed that the nanoparticles were uniform in size and spherical or spheroidal.Conclusion The prescription can be used for the preparation of diosmetin solid lipid nanoparticles,and the process is simple and feasible,stable and feasible.