Rh-D-稀有血型免疫血清学分析及家系调查——附新生儿溶血病报告1例

Serological analysis and family investigation on a rare Rh-D-phenotype:a case report of hemolytic disease of the newborn

目的对1例严重高胆红素血症新生儿及其家系成员进行免疫血清学检测,诊断新生儿溶血病。方法对患儿进行直接抗球蛋白试验,放散试验及游离试验;对患儿母亲进行抗体筛查及抗体鉴定;检测母系家系成员Rh表现型并绘制家系图。结果母亲为Rh-D-稀有血型,产生针对高频抗原的抗体引起患儿溶血。该家系中有6位-D-基因携带者。结论发现了1名Rh-D-稀有血型个体,诊断了1例由高频抗体导致的新生儿溶血病。

Objective To perform the immunoserological testing on a newborn,who developed severe hyperbilirubinemia shortly after birth,and her family members for further diagnosing of hemolytic disease of the newborn(HDN).Methods Direct antiglobulin test,elution test and indirect antiglobulin test were performed on the newborn,and antibody screening and identification were carried out on her mother afterwards.A pedigree chart was drawn based on the Rh phenotypes of maternal family members.Results Rare Rh-D-phenotype was identified in this mother and antibodies against Rh17 was considered as the cause of the newborn′s hyperbilirubinemia.Six-D-gene carriers were found in the family.Conclusion A case of HDN caused by antibodies against Rh17 was diagnosed in the infant born to the mother with rare Rh-D-phenotype.