乳腺浸润性导管癌组织中HPV16/18型感染和Rb蛋白的表达状态及相关性研究

Expression and correlation of HPV16/18 infection and Rb protein in invasive ductal carcinoma of breast

目的探讨乳腺良恶性病变中人乳头状瘤病毒(HPV)16/18型感染和Rb蛋白的表达状态及其相关性。方法收集2016年1月—2017年12月广西壮族自治区柳州市人民医院乳腺外科乳腺手术切除标本为研究对象,其中浸润性导管癌(IDC)68例为IDC组、乳腺不典型导管上皮增生(ADH)30例为ADH组、乳腺纤维腺瘤旁正常乳腺组织30例为正常乳腺组。原位杂交法检测HPV16/18 DNA,免疫组织化学检测Rb蛋白的表达情况。结果IDC组中HPV16/18 DNA阳性率高于正常乳腺组和ADH组;Rb蛋白阳性表达率低于正常乳腺组和ADH组(均P<0.05)。三组HPV16/18 DNA感染与Rb蛋白表达均呈负相关性(r=-0.802、-0.774、-0.802,均P<0.05)。IDC患者HPV16/18DNA、Rb蛋白的表达与组织学分级、淋巴结转移和pTNM分期密切相关(P<0.01)。结论HPV16/18型感染和Rb蛋白的表达缺失可能涉及人乳腺癌的发生、发展过程。

Objective To investigate the expression of human papilloma virus(HPV)16/18 and Rb protein in benign and malignant breast lesions and its correlation.Methods From January 2016 to December 2017,the surgical breast surgically resected specimens in Liuzhou People′s Hospital of Guangxi Zhuang Artonomous Region as the research object,68 cases with invasive ductal carcinoma(IDC)of IDC group,30 cases with breast atypical ductal epithelial hyperplasia(ADH)of ADH group,30 cases with normal breast tissue adjacent to breast fibroadenoma of normal breast group.HPV16/18 DNA was detected by in situ hybridization and Rb protein was detected by immunohistochemistry.Results HPV16/18 DNA positive rate in IDC group was higher than that in normal breast group and ADH group;the positive Rb protein expression rate was lower than that in normal breast group and ADH group(all P<0.01).The expression of HPV16/18 DNA in each group was negatively correlated with the expression of Rb protein(r=-0.802,-0.774,-0.802,all P<0.05).The expression of HPV16/18 DNA and Rb protein in IDC patients was closely related to histological grade,lymph node metastasis and pTNM stage(P<0.01).Conclusion The HPV16/18 infection and Rb protein expression deletion may be involved in the occurrence and development of breast cancer.