自噬相关基因在系统性硬化症患者外周血单个核细胞中的表达及临床意义

Expression and clinical significance of autophagy-related genes in peripheral blood mononuclear cells of systemic sclerosis

目的探讨自噬相关基因LC-3mRNA、Beclin-1mRNA、Agt-3mRNA、Agt-5 mRNA、Agt-12mRNA和Agt-16L1 mRNA在系统性硬化症(systemic sclerosis,SSc)患者外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中的表达及临床意义。方法选取2018年4月--2019年3月于川北医学院附属医院就诊的SSc患者(SSc组)29例和年龄、性别相匹配的对照组(健康体检者)29例,采用RT-PCR检测自噬相关基因在PBMC中的表达水平。运用SPSS 19.0统计学软件比较组间自噬相关基因的表达,分析自噬相关基因与临床资料之间的关系,P<0.05为差异有统计学意义。结果①与对照组相比,自噬相关基因LC-3.Beclin-1和Agt-3在SSc组中表达增加[LC-3:0.77(0.51,1.00)×10-3 vs.0.46(0.37,0.63)×10-3;Beclin-1:6.13(5.01,7.88)×10-3 vs.4.76(3.43,5.34)×10-3;Agt-3:15.67(11.52,23.66)×10-3vs.8.65(7.44,11.23)×10-3],而自噬相关基因Agt-5、Agt-12及Agt-16L1组间差异无统计学意义;②LC-3在抗核小体抗体、抗SSA/Ro抗体阳性患者中表达更高;③LC-3的相对表达量与SSc患者的年龄ESR呈正相关,r值分别为0.6620.428(均P<0.05)。结论自噬相关基因在SSe患者外周血单个核细胞中表达增加,并与年龄、ESR、抗体相关,提示自噬是SSc发病机制中的一个重要特征。

Objective To investigate the expression and clinical significance of autophagy-related genes LC-3 mRNA, Beclin-1 mRNA, Agt-3 mRNA, Agt-5 mRNA, Agt-12 mRNA and Agt-16 L1 mRNA in peripheral blood mononuclear cell(PBMC) of systemic sclerosis(SSc). Methods Twenty-nine cases of SSc and 29 cases of normal control were received from the Affiliated Hospital of North Sichuan Medical College, and autophagy-related genes were detected by RT-PCR. SPSS 19.0 statistical software was used to compare the expression of autophagy-related genes between groups and analyze the relationship between autophagy-related genes and clinical data, P<0.05 was considered statistically significant. Results(1)LC-3, Beclin-1, and Agt-3 were highly expressed in SSc compared with normal control [LC-3:0.77(0.51,1.00)×10-3 vs. 0.46(0.37,0.63)×10-3;Beclin-1: 6.13(5.01,7.88) ×10-3 vs. 4.76(3.43,5.34)×10-3;Agt-3:15.67(11.52,23.66)×10-3 vs. 8.65(7.44,11.23)×10-3], however Agt-5, Agt-12 and Agt-16 L1 of autophagy-related genes were not statistically significant.(2)LC-3 high expressed in SSc with the positive of anti-nucleosome antibody and the positive of anti-SSA/Ro antibody.(3)LC-3 was positively correlated with Age and ESR, and the values of r were 0.662, 0.428(all P<0.05). Conclusion Autophagy-related genes were increased in PBMC of SSc, and were correlated with age, ESR and autoantibody, suggested that autophagy is a key feature in the pathogenesis of systemic sclerosis.