非小细胞肺癌患者血清中lncRNA GHET1的表达及临床意义

Expression and clinical significance of serum lncRNA GHET1in patients with non-small cell lung cancer

目的探讨非小细胞肺癌(NSCLC)患者血清中lncRNA GHET1的表达及其临床意义。方法收集我院新确诊的50例非小细胞肺癌患者及40例健康对照者血清标本,采用qRT-PCR法检测血清中lncRNA GHET1的表达,并分析其表达水平与临床病理因素及化疗敏感性的关系。结果NSCLC病人血清中GHET1表达水平明显升高(P<0.05),ROC曲线分析结果表明GHET1的水平对NSCLC具有较好的诊断价值(AUC=0.767,P<0.05)。血清GHET1表达水平与NSCLC患者临床资料比较发现,NSCLC病人血清中GHET1表达量与NSCLC肿瘤大小和TNM分期密切相关(P<0.05),且化疗耐药组患者血清GHET1水平明显高于化疗敏感组(P<0.05)。结论NSCLC病人血清中lncRNA GHET1高表达且其表达水平可作为诊断、提示疾病进展和化疗耐药的生物学标志物,具有潜在的临床应用价值。

Objective To investigate the expression of lncRNA GHET1in serum of patients with non-small cell lung cancer(NSCLC)and discuss its clinical significance.Methods The serum samples of 50patients with non-small cell lung cancer and 40healthy controls were collected in our hospital,the expression of lncRNA GHET1in serum was detected by qRT-PCR,and the relationship between GHET1expression and clinicopathological factors or chemosensitivity was analyzed.Results The expression of GHET1in serum of NSCLC patients was significantly increased(P<0.05).The ROC curve analysis showed that the level of GHET1had a good diagnostic value for NSCLC(AUC=0.767;P<0.05).The expression of GHET1in serum of NSCLC patients is closely related to NSCLC tumor size and TNM stage(P<0.05),and the serum GHET1level in chemotherapy-resistant group is significantly increased than that in chemotherapy-sensitive group(P<0.05).Conclusion The expression of GHET1in serum of patients with NSCLC was increased and can be used as a biological marker for diagnosis,suggesting disease progression and chemotherapeutic resistance,and has potential clinical application value.