不同丛枝菌根真菌组合与接种时期对滇重楼幼苗根际土壤理化性质与微生物数量的影响

Effect of Different Arbuscular Mycorrhizal Fungi Combinations and Inoculation Periods on Rhizosphere Soil Physicochemical Properties and Microbial Quantity of Paris polyphylla var.yunnanensis Seedlings

目的:研究不同组合丛枝菌根(arbuscular mycorrhiza,AM)真菌在不同接种时期对滇重楼幼苗根际土壤理化性质与微生物数量的影响,为培育高品质的滇重楼奠定基础。方法:采用室温盆栽接种试验及土壤农化分析方法,对不同AM真菌组合及不同接种时期侵染的滇重楼幼苗根际土壤中的孢子密度、侵染率、根围微生物数量、土壤养分及土壤酶活性等进行分析。结果:不同AM真菌处理组在不同接种时期的侵染率均在80%以上,孢子密度在部分时期高于同时期的对照组(CK),AM真菌与滇重楼幼苗根系共生关系良好。土壤中氮含量降低,速效磷、速效钾含量及土壤pH提高,1年生滇重楼实生苗与野生种源滇重楼幼苗土壤养分较高。土壤微生物数量整体表现出细菌>放线菌>真菌,S3,S5和S8的AM真菌组合的2年生实生苗微生物群落对土壤结构较好,生物量碳的含量较高。土壤中磷酸酶和蛋白酶活性增长率较高,过氧化氢酶活性增长率最低,S2~S6处理组的野生种源滇重楼幼苗和1年生滇重楼实生苗土壤酶活性最佳。结论:不同AM真菌处理组及不同接种时期对滇重楼幼苗根际土壤理化性质与微生物数量存在一定的影响,为人工栽培滇重楼提供了技术基础。

Objective:To study the effects of different arbuscular mycorrhizal(AM)fungi combinations on rhizosphere soil physicochemical properties and microbial quantity in the seedlings of Paris polyphylla var.yunnanensis at different inoculation periods,so as to lay a foundation for cultivating high-quality P.polyphylla var.yunnanensis.Method:The spore density,infection rate,nutrient and enzyme activity in the soil around the roots of P.polyphylla var.yunnanensis seedlings under different AM fungi combinations and different inoculation periods were analyzed by the greenhouse pot inoculation trials and soil agrochemical analysis methods.Result:The infection rate of different AM fungi treatment groups was more than 80%in different inoculation periods,and the spore density was higher than control(CK)group in some periods.It reflected that the relationship between AM fungi and roots of Paris polyphylla seedlings was favorable.The content of nitrogen in the soil decreased,but the content of available P,available K and soil pH increased.The soil nutrients in the cultivar one-year seedlings and wild seedlings were higher.The total number of soil microorganisms showed that bacteria>actinomycetes>fungi.The cultivar two-year seedlings with AM fungi combinations of S3,S5 and S8 had better soil structure and higher biomass carbon content.The growth rates of phosphatase and protease activity were higher in the soil,but catalase activity was lowest.In the treatment groups S2,S3,S4,S5 and S6,the soil enzyme activities of the wild seedlings and one-year-old seedlings were best.Conclusion:Different AM fungal treatment groups and different inoculation periods had certain effects on rhizosphere soil physicochemical properties and microbial quantity in the seedlings of P.polyphylla var.yunnanensis,which provided a technical basis for the cultivation of P.polyphylla var.yunnanensis.