BCA-1通过ErK信号通路促进高糖环境下人脂肪间充质干细胞增殖和迁移

BCA-1 promotes the proliferation and migration of human adipose-derived mesenchymal stem cells in high glucose environment through ErK signal pathway

目的探讨高糖环境下B淋巴细胞化学引诱物(BCA-1)对人脂肪间充质干细胞(hAdMSCs)增殖和迁移的影响及其分子机制。方法在细胞高糖模型下,设立高糖对照组、高糖BCA-1组、高糖Erk抑制剂组;正常环境下为正常对照组。CCK8实验检测各组hAdMSCs增殖的吸光度值(A值),Transwell细胞迁移实验检测各组hAdMSCs的迁移率,酶联免疫(ELISA)方法检测各组hAdMSCs中PI3K、MAPK和VEGF蛋白的表达。结果细胞高糖环境下,高糖BCA-1组hAdMSCs的A值、迁移率均升高,差异有高度统计学意义(P<0.01);高糖BCA-1组hAdMSCs的PI3K、MAPK、VEGF蛋白含量高表达,差异有高度统计学意义(P<0.01);与高糖BCA-1组hAdMSCs比较,高糖Erk抑制剂组hAdMSCs的A值和迁移率均明显降低,差异有高度统计学意义(P<0.01),高糖Erk抑制剂组hAdMSCs的PI3K、MAPK和VEGF蛋白表达均分别低于高糖BCA-1组,差异有统计学意义(P<0.05)。结论高糖环境下,高糖BCA-1通过PI3KErk-MAPK信号通路,上调hAdMSCs旁分泌VEGF蛋白,促进hAdMSCs增殖和迁移。

Objective To investigate the effect of B lymphocyte chemoattractant(BCA-1)on the proliferation and migration of human adipose-derived mesenchymal stem cells(hAdMSC)in high glucose environment and its molecular mechanism.Methods The high glucose control group,the high glucose BCA-1 group and the high glucose Erk inhibitor group were established with the cells in high glucose model.Normal control group was under normal environment.CCK8 test was used to detect the absorbance value(A value)of proliferation of hAdMSCs in each group,Transwell cell migration assay was used to detect the mobility of hAdMSCs in each group,and ELISA was used to detect the expression of phosphatidylinositol 3-kinase(PI3K),mitogenactivated protein kinase(MAPK)and vascular endothelial growth factor(VEGF)proteins in hAdMSCs in each group.Results With cells in high glucose environment,the A value and mobility of the hAdMSCs in the high glucose BCA-1 group were increased,and the differences were statistically significant(P<0.01).The contents of PI3K,MAPK and VEGF protein in the hAdMSCs in the high glucose BCA-1 group were highly expressed,and the differences were statistically significant(P<0.01).Compared with those of the hAdMSCs in the high glucose BCA-1 group,the A value and mobility of the hAdMSCs in the high glucose Erk inhibitor group both decreased significantly,and the differences were statistically significant(P<0.01).The expressions of PI3K,MAPK and VEGF proteins in the hAdMSCs in the high glucose Erk inhibitor group were lower than those in the high glucose BCA-1 group respectively,and the differences were statistically significant(P<0.05).Conclusion In high glucose environment,high glucose BCA-1 up-regulates the paracrine of VEGF protein by hAdMSCs through PI3K-Erk-MAPK signal pathway and promotes the proliferation and migration of hAdMSCs.