microRNA-200b调节口腔鳞癌E-钙黏着蛋白的研究

microRNA-200b Regulating E-cadherin in Oral Squamous Cell Carcinoma

目的通过口腔鳞癌细胞中过表达microRNA-200b,检测口腔鳞癌细胞E-钙黏着蛋白表达是否有所上调;并检测E-钙黏着蛋白表达上调对口腔鳞癌细胞生物学特性的影响。方法通过目的基因过表达慢病毒载体构建(has-miR 200b-LV),转染舌癌细胞株Tca8113,检测转染后舌癌细胞株Tca8113的E-钙黏着蛋白的表达及转染后舌癌细胞株Tca8113的侵袭和转移情况。结果实验组通过Real-time PCR数值分析显示miR-200b基因表达定量分析平均值比较,组间差异有统计学意义(P<0.05);转染后的Tca8113细胞,通过Real-time PCR数值分析显示实验组中E-cadherin基因表达定量分析平均值比较,组间差异有统计学意义(P<0.05);Transwell侵袭实验中,空白组和对照组穿膜细胞数分别为(29.3±4.5)和(20.2±3.3),与实验组(4.3±2.1)比较,差异有统计学意义(P<0.05);生长曲线显示microRNA-200b过表达病毒感染的肿瘤细胞生长速度明显减慢。结论microRNA-200b的表达可以调控E-钙黏着蛋白的表达,E-钙黏着蛋白的高表达可以抑制肿瘤的侵袭和转移。

Objective To detect whether the expression of E-cadherin in oral squamous cell carcinoma cells is up-regulated by overexpressing microRNA-200b in oral squamous cell carcinoma cells;and to detect the effect of up-regulation of E-cadherin expression on the biological characteristics of oral squamous cell carcinoma cells influences.Methods The target gene overexpression lentiviral vector(has-miR 200b-LV)was constructed and transfected into the tongue cancer cell line Tca8113.The expression of E-cadherin in the tongue cancer cell line Tca8113 after transfection and the tongue cancer cell line after transfection were detected.Results The real-time PCR numerical analysis of the experimental group showed that the average value of miR-200b gene expression quantitative was compared,and the difference between the groups was statistically significant(P<0.05);the transfected Tca8113 cells passed Real-time Time PCR numerical analysis showed that the average value of E-cadherin gene expression quantitative analysis in the experimental group was(compared),and the difference between the groups was statistically significant(P<0.05);in the Transwell invasion experiment,the blank group and the control group had permeabilized cells numbers were(29.3±4.5)and(20.2±3.3),which were statistically different from the experimental group(4.3±2.1)(P<0.05);the growth curve showed the growth rate of microRNA-200b overexpression virus-infected tumor cells was significantly slowed down.Conclusion The expression of microRNA-200b can regulate the expression of E-cadherin,and the high expression of E-cadherin can inhibit tumor invasion and metastasis.