同位素内标-UPLC-MS/MS法检测易拉罐啤酒中双酚A含量

Determination of Bisphenol A in Canned Beer by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry based on Isotope Internal Standard

目的为了提高金属易拉罐啤酒中双酚A(BPA)的检测能力,建立一种基于同位素内标的超高效液相色谱-串联质谱分析方法。方法样品经超声处理后除去CO2,随后添加内标物BPA-13C12,用超高效液相色谱-串联质谱法进行检测分析,采用内标法定量。结果经同位素内标标准曲线校正,方法的定量限为1.00μg/L,线性方程为y=1.73x+0.206,线性相关系数为0.9992,相对标准偏差为4.68%6.63%,加标回收率为91.0%~109%。使用该方法对厦门市市售6种易拉罐啤酒样品进行了BPA污染物迁移量检测,结果显示6种啤酒中均检出BPA,平均质量浓度为1.48μg/L。结论此方法具有前处理简便快捷、检测限高、灵敏度高、准确度好等特点,能够满足金属易拉罐啤酒中低含量BPA的检测要求。

The work aims to establish a method based on isotope internal standard by ultra performance liquid chromatography-tandem mass spectrometry(UPLC/MS-MS)to enhance the determination ability of bisphenol A(BPA)in canned beer.The canned beer samples were mixed with BPA-13C12 after ultrasonic treatment,and then analyzed by UPLC/MS-MS and quantified by isotope-labelled internal standard.Quantified by the calibration curve of isotope-labelled internal standard,the detection limitation of the method was 1.00μg/L.The linear equation was y=1.73 x+0.206,the correlation coefficient was 0.9992,and the spiked recoveries of BPA was 91.0%to 109%with RSDs ranging from 4.68%to 6.63%.Then,the method was applied to determine the pollutant migration of BPA in 6 canned beers from Xiamen local market.The results showed that BPA was found in all samples with the average concentration of 1.48μg/L.The method has the advantages of simple and fast pretreatment,low detection limitation,high sensitivity and good accuracy,which can satisfy the requirement of detecting low BPA concentration in canned beer.