酒中吖啶含量检测方法的比较研究

Comparative Study on Determination Methods of Acridine in Alcoholic Beverages

采用液相色谱-荧光法、液相色谱-紫外吸收法以及液相色谱-单四级杆质谱联用法分别对不同类型的酒中吖啶的含量进行测定,并对其灵敏度、准确度、精密度和样品加标回收进行分析。结果表明,液相色谱-荧光法最低检出限为0.083μg/L,线性范围为100 ng/L^100μg/L,线性相关系数为0.9999,相对误差为-1.1%,样品加标回收率为80%~118%;液相色谱-紫外吸收法最低检出限为2.2μg/L,线性范围为10μg/L^10 mg/L,线性相关系数为0.9999,相对误差为9.0%,样品加标回收率为42%~102%;液相色谱-单四级杆质谱联用法最低检出限为4.7μg/L,线性范围为10~600μg/L,线性相关系数为0.9995,相对误差为-9.3%,样品加标回收率为68%~92%。3种方法的准确度和精密度均较好,但灵敏度上以液相色谱-荧光法最佳,在针对不同样品时各检测方法有其独特的优势,实验室可根据实际使用需求选择有效的检测方法。

In this study,liquid chromatography-fluorescence(HPLC-FLD),liquid chromatography-ultraviolet absorption(HPLC-UV)and liquid chromatography-single quadrupole mass spectrometry(HPLC-MS)were used to determine the content of acridine in different types of alcoholic beverages,and the sensitivity,accuracy,precision and sample spiked recovery of the three methods were analyzed.The results showed that the minimum detection limit of HPLC-FLD was 0.083μg/L,the linear range was 100 ng/L to 100μg/L,the linear correlation coefficient was 0.9999,the relative error was-1.1%,and the sample spiked recovery rate was 80%to 118%;the minimum detection limit of HPLC-UV was 2.2μg/L,the linear range was 10μg/L to10 mg/L,the linear correlation coefficient was 0.9999,the relative error was 9.0%,and the sample spiked recovery rate was 42%to 102%;the minimum detection limit of HPLC-MS was 4.7μg/L,the linear range was 10μg/L to 600μg/L,the linear correlation coefficient was 0.9995,the relative error was-9.3%,and the sample spiked recovery rate was 68%to 92%.The accuracy and precision of the three methods were good,while HPLC-FLD had the best sensitivity.Each method has its unique advantages for different samples,and the laboratory can choose an effective one according to actual needs.