前列爽防治前列腺增生作用机理初探

Mechanism of Qianlieshuang on Preventing and Treating Benign Prostatic Hyperplasia

目的:评价前列爽对前列腺增生的防治作用并探讨其可能的作用机制。方法:以雄性小鼠和大鼠复制前列腺增生模型,灌胃前列爽(小鼠2.0、4.0、8.0 g/kg,大鼠1.5、3、6 g/kg),取前列腺称重计算其指数。评价其对前列腺指数、腺上皮面积、前列腺病理的影响;另取连续造模5 w,连续给药5 w大鼠,末次给药半小时后,测定基础膀胱内压,静脉注射盐酸去氧肾上腺素2.0 mg/kg造模,观察静脉注射药物后膀胱内压的变化,取前列腺称重计算其指数。结果:小鼠在注射丙酸睾丸酮3 w后,与正常对照组比较,模型对照组前列腺指数显著升高(P<0.01);与模型对照组比较,前列爽8.0、4.0、2.0 g/kg均可显著对抗丙酸睾酮的作用,显著降低其前列腺指数(P<0.05或P<0.01)。大鼠在注射丙酸睾酮注射液5.0 mg/kg 8 w后,与正常对照组比较,模型对照组前列腺指数、腺上皮所占的比例均显著升高(P<0.05或P<0.01);与模型对照组比较,模型大鼠给予前列爽3.0 g/kg、6.0 g/kg可显著降低前列腺指数(P<0.05),前列爽6.0 g/kg还可显著降低其腺上皮所占的面积比例。大鼠在连续注射丙酸睾酮注射液5 mg/kg 5 w后,与正常对照组比较,模型对照组前列腺指数显著增加(P<0.01),膀胱内压对去氧肾上腺素的敏感性显著增加(P<0.01);与模型对照组比较,前列爽3.0 g/kg和6.0 g/kg可以显著对抗丙酸睾酮增加前列腺指数的作用,前列爽6.0 g/kg还可显著对抗去氧肾上腺素致前列腺增生大鼠膀胱内压升高(P<0.05或P<0.01)。结论:前列爽对前列腺增生有较好的防治作用,既能降低前列腺指数,改善前列腺增生所致的静力性膀胱出口梗阻;也能对抗去氧肾上腺素,降低大鼠膀胱内压,缓解前列腺增生所致的动力性膀胱出口梗阻。其作用机理可能与其对抗雄激素、肾上腺素能α受体有关,详细相关机制有待进一步深入研究。

Objective:To evaluate the prevention and treatment effect of Qianlieshuang on prostatic hyperplasia and its mechanism.Methods:Prostatic hyperplasia mice model and rats model were established.After orally administrated in mice(8.0,4.0,2.0 g/kg doses)and rats(1.5,3,6 g/kg doses),prostates were collected and weighed,then the effect of Qianlieshuang on the prostate index,the area of glandular epithelium and pathological changes were studied.Rats with modeling for 5 weeks and administration for 5 weeks were taken,0.5 h after last administration,to measure their intravesical pressures before and after 2.0 mg/kg phenylephrine hydrochloride was intravenously injected,then prostates were collected to calculate the index.Results:After 3 weeks of testosterone propionate injection into mice,compared with the control group,the prostate index was increased in the model group(p<0.01).Compared with the model group,the prostate indexes were significantly decreased in Qianlieshuang groups(8.0,4.0,2.0 g/kg),which suggested an anti-prostatic hyperplasia effect(p<0.05 or p<0.01).After 8 weeks of injection with 5.0 mg/kg testosterone propionate in rats,compared with the control group,the prostate index and the area of glandular epithelium were significantly increased in the model group(p<0.05 or p<0.01).Compared with the model group,the prostate indexes were significantly decreased in 3.0 g/kg and 6.0 g/kg Qianlieshuang groups(p<0.05),and the area of glandular epithelium was significantly decreased in 6.0 g/kg group.After 5 weeks of 5.0 mg/kg testosterone propionate injection,compared with the control group,the prostate index was significantly increased in the model group(p<0.01),the sensitivity of intravesical pressure to phenylephrine was induced(p<0.01).Compared with the model group,the prostate indexes were significantly decreased in Qianlieshuang groups(3.0 g/kg,6.0 g/kg),also the sensitivity of intravesical pressure to phenylephrine was reduced in 6.0 g/kg Qianlieshuang group(p<0.05 or p<0.01).Conclusion:Qianlieshuang has the good preventative and therapeutic effects on prostatic hyperplasia.It may decrease the prostate index and improve the static bladder outlet obstruction caused by prostatic hyperplasia.It can also decrease intravesical pressure in rats and relieve dynamic bladder outlet obstruction.Its mechanism might be related to its anti-androgen and adrenoreceptorα,and further research is needed.