苏合香对脑缺血再灌注损伤大鼠的脑保护及血脑屏障开放作用研究

Experimental study of Storax on brain protection and blood-brain barrier permeability of rats with cerebral ischemia reperfusion injury

目的探究开窍药苏合香对脑缺血再灌注大鼠的脑损伤及血脑屏障通透性的影响。方法健康成年雄性Wistar大鼠随机分为3组:假手术组、模型组、苏合香(0.4 g/kg)组。线栓法建立短暂性大脑中动脉闭塞模型,于脑缺血2 h后ig给药,检测大鼠脑缺血后神经功能评分,计算大鼠脑含水量及脑梗死率,检测大鼠血常规及鼠尾出血时间。应用伊文思蓝染色法检测大鼠生理状态以及脑缺血后6、12、24、48、72 h血脑屏障通透性的变化。采用凝血四项试剂盒检测大鼠脑缺血6、12、24、48、72 h的凝血功能。结果与模型组比较,苏合香显著改善脑缺血大鼠神经行为学评分(P<0.05),显著降低脑梗死率(P<0.05),显著延长鼠尾出血时间(P<0.05),但对脑含水量及血常规无明显影响;血脑屏障通透性实验结果显示,苏合香能够开放正常大鼠血脑屏障(P<0.01);与模型组比较,苏合香能增强脑缺血再灌注大鼠血脑屏障通透性,且缺血48 h时最为显著(P<0.01);另外苏合香可显著延长脑缺血24 h时大鼠凝血酶原时间(PT)、凝血活酶时间(TT),降低纤维蛋白原(FIB)含量(P<0.05)。结论苏合香对脑缺血再灌注损伤大鼠具有明显脑保护作用,同时可能通过影响凝血功能增加正常及缺血状态下血脑屏障通透性,为苏合香脑保护作用研究提供了新的线索。

Objective To explore the effects of storax on brain protection and blood-brain barrier(BBB) permeability of rats with cerebral ischemia-reperfusion(I/R) injury.Methods Wistar male rats were randomly divided into three groups: Sham group, model group, and storax(0.4 g/kg) group.The model of transient middle cerebral artery occlusion(tMCAO) was established by sutureoccluded method, rats were ig with drugs two hours after cerebral ischemia, then neurological function scores, cerebral infarction rate and cerebral edema of rats after cerebral ischemia were measured, and the blood routine and the tail bleeding time of the rats were measured as well.Evans blue staining method was used to detect the physiological status of rats and the changes in BBB permeability of 6, 12, 24, 48, and 72 h cerebral ischemia.Coagulation four test kit were used to detect the coagulation function of rats with cerebral ischemia at 6, 12, 24, 48, 72 h.Results Compared with model group, storax can significantly improve the neurobehavioral scores(P < 0.05), reduce the rate of cerebral infarction(P < 0.05), and prolong the bleeding time of rats tail(P <0.05), but it had no obvious effect on the brain water content and blood test;Storax can increase the BBB permeability of physiological rats with the increasing time and compared with the model group, the permeability of BBB could be enhanced by storax, which was most significant at 48 h(P < 0.01).In addition, storax can prolong the prothrombin time(PT) and prothrombin time(TT), and decreased the fibrinogen(FIB) content after ischemic stroke of rats at 24 h(P < 0.05).Conclusion Storax has obvious neuroprotective effect on rats with cerebral I/R injury, and increase the permeability of the BBB under normal and ischemic conditions, which may be related to prolonged blood coagulation and anti-platelet aggregation.