HLA-DRB1等位基因与广东汉族人群甲真菌病的相关性研究

Relationship between HLA-DRB1 alleles and onychomycosis in Chinese Han population in Guangdong province

目的:探讨人类白细胞抗原HLA-DRB1等位基因与广东汉族人群甲真菌病的关联性。方法:纳入64例广东汉族甲真菌病患者(包括10例红色毛癣菌甲癣患者)以及64例健康对照者,采用聚合酶链式反应-序列特异性引物(PCR-SSP)技术,对研究对象全血基因DNA进行HLA-DRB等位基因分型,比较等位基因频率,分析甲真菌病与HLA基因多态性的关系。结果:甲真菌病患者HLA-DRB1*10基因频率高于健康对照组(X^2=5.10,P <0.05)。红色毛癣菌感染的甲癣患者组HLA-DRB1*12基因频率低于健康对照组(X^2=4.70,P <0.05)。结论:HLA-DRB1*10等位基因可能是广东汉族人群甲真菌病遗传易感基因,而HLA-DRB1*12等位基因可能是红色毛癣菌感染的甲真菌病患者的保护性基因,HLA基因多态性与甲真菌病的发生可能存在遗传免疫关联性。

Objective:To investigate the relationship between polymorphism of human leukocyte antigen(HLA)-DRB1 alleles and onychomycosis in Chinese Han population in Guangdong province.Methods:Polymerase chain reaction-sequence specific primers(PCR-SSP)technique was adopted to detect the allele frequencies of HLA-DRB1 in 64 cases of onychomycosis,including 10 cases of Trichophyton rubrum infection,and 64 healthy controls.The differences in allele frequencies were compared between patients and controls.Results:The allele frequencies of HLA-DRB1*10 in Han patients with onychomycosis were higher than that in the controls(x^2=5.10,P<0.05).In contrast,the allele frequencies of HLA-DRB1*12 in Han patients with trichophyton rubrum infection were lower than that in the controls(x^2=4.70,P<0.05).Conclusions:HLA-DRB1*10 alleles may be the susceptibility gene of onychomycosis in Chinese Han population in Guangdong province.HLA-DRB1*12 alleles could prevent the development of onychomycosis caused by trichophyton rubrum.HLA alleles could be immunogenetically associated with onychomycosis.