银屑病皮损间充质干细胞对人角质形成细胞增殖及凋亡的影响

Effects of psoriatic lesional mesenchymal stem cells on proliferation and apoptosis of human keratinocytes

目的研究银屑病皮损间充质干细胞(dermis mesenchymal stem cells,DMSCs)对正常人表皮角质形成细胞(human normal keratinocytes cells,KCs)增殖及凋亡的影响。方法体外分离培养DMSCs和KCs,设3个组:KCs单纯培养组、健康对照组(正常DMSCs+KCs)和试验组(皮损DMSCs+KCs)。首先用皮损DMSCs上清孵育KCs,用CCK-8检测细胞存活率;再用Transwell培养96 h后,用CCK-8检测细胞存活率,流式细胞仪检测细胞凋亡,酶联免疫吸附试验(ELISA)检测细胞培养上清中转化生长因子(TGF)-β1浓度。结果共培养组与KCs单纯组相比细胞增殖和凋亡均增多;与健康对照组相比,患者组角质细胞增殖增多,凋亡减少,TGF-β1水平下降。结论银屑病皮损DMSCs可以促进角质细胞的增殖,同时促细胞凋亡能力减弱,可能与TGF-β1水平分泌异常有关。

Objcetive To study the effect of mesenchymal stem cells from psoriatic lesions on proliferation and apoptosis of keratinocytes.Methods Keratinocytes cells were isolated and cultured in vitro,and divided into three groups:KCs monoculture,coculture with control MSCs and co-culture with psoriatic MSCs.Firstly,KCs was incubated with DMSCs supernatant from skin lesions,then,the cells were co-cultured by transwell plate.After 96 hours,the cells viability was detected by CCK-8,apoptosis was detected by flow cytometry,and the concentration of TGF-β1 in the cell culture supernatant was detected by ELISA.Results Cell proliferation and apoptosis were both increased in the co-culture group compared with the KCs monoculture group.In addition,compared with the healthy control group,the proliferation of KCs treated with psoriatic DMSCs was increased,but the apoptosis and the TGF-β1 level were decreased.Conclusion Psoriatic DMSCs can promote the proliferation of keratinocytes and weaken the cell apoptosis ability which may be related to abnormal secretion of TGF-β1.