摘要
为青海湖裸鲤人工扩繁和分子遗传育种研究提供理论基础,采用RACE和RT-PCR克隆青海湖裸鲤foxk1全长cDNA序列,对其进行生物信息分析和功能分析。结果表明:青海湖裸鲤foxk1全长cDNA序列为2251bp,包含1944-bp开放阅读框,编码647个氨基酸;克隆的foxk1与其它脊椎动物的氨基酸序列同源性为68.2%~94.4%,亲缘性相近;foxk1与其邻近基因gnal2、sdk1、mmd2的共线性在整个脊椎动物中高度保守;青海湖裸鲤foxk1高表达于垂体和性腺,中量表达于脑,微量表达于鳃和心脏;青海湖裸鲤foxk1在3月和6月卵巢中低表达,在9月及后期卵巢中持续高表达。
In order to provide a theoretical basis for artificial propagation and molecular genetic breeding of Gymnocypris przewalskii,the full-length cDNA of foxk1 was cloned fromG.przewalskii by using RT-PCR and RACE,and the biological information analysis and functional analysis were conducted as well.Results:The cloned foxk1 gene was 2251 bp,containing a 1944-bp open reading frame which encoded 647 amino acids.The alignment showed that cloned foxk1 was highly conserved with other vertebrates with the similarity between 68.2%-94.4%.Syntenic analysis showed that foxk1 and its adjacent gene gnal2,sdk1,mmd2 were highly conserved.RT-PCR displayed that foxk1 was expressed highly in the pituitaries and gonads,moderately in the brains,rarely in the gills and hearts.Real-time PCR analysis revealed that foxk1 was expressed lowly in March and June,highly in the ovaries in September and afterwards.
作者
闵倩雯
张显波
周其椿
李建光
曾圣
陈飞雄
杨兴
MIN Qianwen;ZHANG Xianbo;ZHOU Qichun;LI Jianguang;ZENG Sheng;CHEN Feixiong;YANG Xing(Guizhou Fisheries Research Institute,Guizhou Academy of Agriculture Sciences,Guiyang,Guizhou 550025;Guizhou Technology Center for Special Fishery Engineering,Guiyang,Guizhou 550025,China)
出处
《贵州农业科学》
CAS
2020年第10期73-76,共4页
Guizhou Agricultural Sciences
基金
国家农业部项目“国家特色淡水鱼产业技术体系”(CARS-46),“国家大宗淡水鱼产业技术体系”(9CARS-46-49)
贵州省科技厅支撑项目“青海湖裸鲤引种驯养及山区流水健康养殖配套技术”[黔科合支撑(2019)2349]
贵州省农业委员会项目“贵州省特色水产产业技术体系”(GZ-CYTX2018-011)
贵州省农业科学院青年基金项目“青海湖裸鲤引种、驯养及与其生长性状相关的分子标记研究”[黔农科院青年基金(2017)21]
贵州省农业科学院2020年省财政科研专项资金项目[黔农科院种质资源(2020)05]。