小红蒜总萘醌质量标准的研究

Quality standard for total naphthoquinones in Eleutherine plicata

目的建立小红蒜总萘醌的质量标准。方法TLC、HPLC法分别定性鉴别、定量测定异红葱乙素、红葱乙素、红葱甲素,2015年版《中国药典》方法检测干燥失重、水分、炽灼残渣、树脂残留物,紫外分光光度法测定总萘醌含有量。结果TLC斑点清晰,分离度良好。3批样品干燥失重、水分、炽灼残渣范围分别为2.34%~4.03%、2.06%~3.56%、0.15%~0.19%,树脂残留物符合相关标准。总萘醌、异红葱乙素、红葱乙素、红葱甲素分别在1~10μg/mL(r=0.9994)、0.00500~0.200 mg/mL(r=0.9997)、0.0144~0.576 mg/mL(r=0.9999)、0.00496~0.198 mg/mL(r=0.9999)范围内线性关系良好,平均加样回收率分别为100.06%(RSD=1.92%)、101.77%(RSD=1.30%)、103.24%(RSD=1.04%)、98.41%(RSD=1.82%)。结论该方法合理可行,可用于小红蒜总萘醌的质量控制。

AIM To establish the quality standard for total naphthoquinones in Eleutherine plicata Herb.METHODS TLC and HPLC were adopted in the qualitative identification and quantitative determination of isoeleutherin,eleutherin and eleutherol,respectively.Chinese Pharmacopoeia(2015 edition)method was used to detect loss on drying,water,ignition residue and resin residue.UV spectrophotometry was applied to determining the content of total naphthoquinones.RESULTS The TLC spots were clear with good separation.In three batches of samples,the ranges of loss on drying,water and ignition residue were 2.34%-4.03%,2.06%-3.56%and 0.15%-0.19%,respectively,and resin residue accorded with relative requirements.Total naphthoquinones,isoeleutherin,eleutherin and eleutherol showed good linear relationships within the ranges of 1-10μg/mL(r=0.9994),0.00500-0.200 mg/mL(r=0.9997),0.0144-0.576 mg/mL(r=0.9999)and 0.00496-0.198 mg/mL(r=0.9999),whose average recoveries were 100.06%(RSD=1.92%),101.77%(RSD=1.30%),103.24%(RSD=1.04%)and 98.41%(RSD=1.82%),respectively.CONCLUSION This reasonable and feasible method can be used for the quality control of total naphthoquinones in E.plicata.