养心氏片对心肌梗死后抑郁大鼠的影响

Effects of Yangxinshi Tablets on rats with myocardial infarction complicated with depression

目的研究养心氏片对心肌梗死后大鼠抑郁的预防作用,并探讨潜在机制。方法将50只大鼠随机分为假手术组、模型组、氟西汀组(10 mg/kg)、养心氏片低剂量和高剂量组(500、1000 mg/kg),每组10只,灌胃给药4周后。模型组、氟西汀组、养心氏组的大鼠进行左冠状动脉前降支结扎制造心肌梗死。造模成功后进行强迫游泳诱发抑郁症。心脏彩超评价心脏功能。行为学实验评估抑郁症状。检测炎症因子和单胺类神经递质探索养心氏片的潜在机制。结果与模型组比较,养心氏组的心功能(LVIDd、LVIDs、LVEDV和LVESV增大,FS和EF减小)改善(P<0.05),抑郁症状(糖水偏爱、不动时间和运动得分)缓解(P<0.05,P<0.01),炎症因子(TNF-α、IL-1β、IL-6水平以及IDO1、KMO、KYNU表达)降低(P<0.05,P<0.01),单胺神经递质(5-HT、ACTH、CORT、NE)上升(P<0.05,P<0.01)。结论养心氏片能有效改善心肌梗死后抑郁症大鼠的心功能和抑郁症状。其机制可能与抵抗炎症和增加单胺神经递质利用有关。

AIM To study the preventive effect of Yangxinshi Tablets on rats with myocardial infarction complicated with depression and to explore the underlying mechanism.METHODS Fifty rats were randomly di-vided into sham-operated group,control group,fluoxetine group(10 mg/kg),low dose and high dose Yangxinshi Tablets groups(500,1000 mg/kg).Ten rats in each group were intragastrically administered for 4 weeks.Rats in the model group,fluoxetine group,Yangxinshi Tablets groups developed myocardial infarction after left anterior de-scending coronary artery ligation,and thereafter forced depression by swimming.The rats were subjected to cardiac function evaluation by cardiac color Doppler ultrasound,depressive symptoms assessment by behavioral experiments,and detection of inflammatory factors and monoamine neurotransmitters to reveal the underlying mechanisms of Yangxinshi Tablets.RESULTS Compared with the model group,Yangxinshi Tablets groups had improved heart function(as revealed by the increased LVIDd,LVIDs,LVEDV and LVESV;decreased FS and EF)(P<0.05),relieved depressive symptoms(in terms of sucrose preference,immobility time and exercise score)(P<0.05,P<0.01),lower inflammatory factors levels(in terms of the levels of TNF-α,IL-1β,IL-6 and the expression of IDO1,KMO,KYNU)(P<0.05,P<0.01),and significantly increased monoamine neurotransmitters(in terms of 5-HT,ACTH,CORT,NE)(P<0.05,P<0.01).CONCLUSION Yangxinshi Tablets can effectively improve the heart function and depressive symptoms in myocardial infarction rats complicated with depression.The mechanism may be related to the improved resistance to inflammation and increased utilization of monoamine neurotransmitters.