UPLC-QTOF-MS法分析沙棘果实、叶和枝的成分

Analysis of constituents in the fruit,leaf and twig of Hippophae rhamnoides by UHPL-QTOF-MS

目的UPLC-QTOF-MS法分析沙棘Hippophae rhamnoides L.果实、叶和枝的成分。方法沙棘果实80%乙醇提取物的分析采用硅胶、大孔树脂、Sephadex LH-20、半制备液相色谱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。通过UPLC-QTOF-MS对沙棘果实、叶和枝不同部位化学成分进行分析。结果从中分离得到6个化合物,分别鉴定为柽柳黄素-3-O-β-D-葡萄糖-7-O-α-L-鼠李糖苷(1)、异鼠李素-3-O-β-D-葡萄糖-7-O-α-L-鼠李糖苷(2)、1-甲基-1,2,3,4-四氢咔啉-3-羧酸(3)、异鼠李素-3-O-β-D-槐二糖-7-O-α-L-鼠李糖苷(4)、芦丁(5)、异鼠李素-3-O-β-D-葡萄糖苷(6)。共鉴定30个化合物,包括8个鞣质类和22个黄酮类,其中25个化合物为3种部位的共有成分。结论化合物3为首次从沙棘属植物中分离得到。该方法准确稳定,重复性好,可用于沙棘的质量控制。

AIM To analyze constituents in the fruit,leaf and twig of Hippophae rhamnoides L.by UHPL-QT-OF-MS.METHODS The 80%ethanol extract from the fruit of H.rhamnoides was isolated and purified by silica,macroporous resin,Sephadex LH-20 and semi-prep HPLC,then the structures of obtained compounds were identi-fied by physicochemical properties and spectral data.Qualitative analysis of chemical constituents in the fruit,leaf and twig of H.rhamnoides was detected by UPLC-QTOF-MS.RESULTS Six compounds were isolated and identi-fied as tamarixetin-3-O-β-D-glucoside-7-O-α-L-rhamnoside(1),isorhamnetin-3-O-β-D-glucoside-7-O-α-L-rham-noside(2),1-methyl-1,2,3,4-tetrahydro carboline-3-carboxylic acid(3),isorhamnetin-3-O-β-D-sophoroside-7-O-α-L-rhamnoside(4),rutin(5),isorhamnetin-3-O-β-D-glucoside(6).Thirty compounds were identified,including eight tannins and twenty-two flavonoids.Twenty-five compounds were common compounds for the three parts of H.rhamnoides.CONCLUSION Compound 3 is isolated from genus Hippophae for the first time.This accurate,stable and reproducible method can be used for the quality control of H.rhamnoides.