干燥方式对丹参茎叶中酚酸类成分及其抗氧化活性的影响

Effects of drying methods on phenolic acids and antioxidant activities of stems and leaves of Salvia miltiorrhiza

目的探究干燥方式对丹参茎叶中酚酸类成分及其抗氧化活性的影响。方法采用福林-酚比色法测定丹参茎叶中总酚酸含有量,HPLC法测定丹酚酸B、迷迭香酸、丹参素、咖啡酸的含有量;采用铁氰化钾还原法、DPPH自由基和超氧阴离子自由基清除法测定其抗氧化活性。结果经真空冷冻干燥的丹参茎叶中总酚酸、迷迭香酸、丹参素和咖啡酸的含有量均最高,分别为112.12、25.94、0.70、0.69 mg/g,干燥方式对丹酚酸B含有量无显著影响。3种干燥方式处理抗氧化活性强弱依次为真空冷冻干燥、自然阴干、恒温鼓风干燥。结论真空冷冻干燥为丹参茎叶的最佳干燥方式。

AIM To investigate the effects of drying methods on phenolic acids and antioxidant activities of stems and leaves of Salvia miltiorrhiza Bge..METHODS The content of total phenolic acids in the stems and leaves of S.miltiorrhiza was determined by Folin-phenol colorimetry,and determination of salvianolic acid B,rosmarinic acid,danshensu and caffeic acid by HPLC.Potassium ferricyanide reduction method,DPPH radical and superoxide anion radical scavenging method were used to determine their antioxidant activity.RESULTS The content of total phenolic acid in the stems and leaves of S.miltiorrhiza was the highest after vacuum freeze drying(112.12 mg/g).The drying method had no significant effect on the content of salvianolic acid B.After vacuum freeze drying,the contents of rosemary acid,danshensu and caffeic acid were the highest in the stems and leaves of S.miltiorrhiza,which were 25.94,0.70,0.69 mg/g,respectively.The order of their antioxidant activity after treatment with three drying methods was vacuum freeze drying,natural drying,constant temperature blast drying.CONCLUSION Vacuum freeze-drying is the best way to dry the stems and leaves of S.miltiorrhiza.