淋球菌对阿奇霉素耐药性及分子流行病学特征

Drug resistance of Gonococci to azithromycin and its molecular epidemiological characteristics

目的连续监测近6年间本院分离的淋球菌对阿奇霉素耐药性及分子流行病学情况,判断本院淋球菌的同源性。方法选取本院2013年-2018年分离的淋球菌培养鉴定,琼脂稀释法测定阿奇霉素的最低抑菌浓度MIC;并使用淋球菌多抗原序列(NG-MAST)进行分型。结果共分离出的283株淋球菌,对阿奇霉素MIC≥1 mg/L即耐阿奇霉素共70株,其中中度耐药(AZM/MLR)即MIC 1 mg/L^64 mg/L共31株,高度耐药(AZM/MLR)即MIC≥64 mg/L 39株;70株阿奇霉素耐药株共鉴定出58种不同ST型别,其中ST1866、ST1766、ST421和ST3102几型为主要型别,无明显优势的ST型别。结论本院近几年淋球菌对阿奇霉素耐药率较高,形势严峻;ST型别分散,遗传背景呈现多样化,覆盖面较广。

Objective To determine the homology of gonococcus in this hospital by continuously monitoring the resistance to azithromycin and molecular epidemiology of gonococcal isolated in the past six years.Methods The minimum antibacterial concentration of azithromycin was determined by agar dilution method.Gonococcal polyantigen sequences( NG-MAST) was used for typing.Results There were 70 strains of azithromycin MIC ≥ 1 mg/L,including 31 strains of moderate resistance( AZM/MLR),namely,MIC 1 mg/L-64 mg/L,and highly resistant( AZM/MLR),that is,MIC ≥64 mg/L( 39 strans);A total of 58 different ST types were identified,of which ST1866,ST1766,ST421,and ST3102 are the dominant types.Conclusion The drug resistance rate of Gonococci to azithromycin is higher in recent years.The ST type is dispersed,and the genetic background is diversified and the coverage is wide.