Linc-ROR调控PI3K/AKT信号通路介导口腔鳞状细胞癌细胞增殖侵袭

Linc-ROR mediates the cell proliferation and invasion of oral squamous cell carcinoma through regulating PI3K/AKT signaling pathway

目的探讨长链非编码RNA ROR(Linc-ROR)在口腔鳞状细胞癌(OSCC)组织和细胞中的表达水平以及对OSCC细胞增殖侵袭的调控。方法选取本院收治的90例OSCC患者的组织标本,采用实时荧光定量PCR分析OSCC组织及细胞中Linc-ROR的相对表达量。构建Linc-ROR过表达的OSC-4细胞系和Linc-ROR敲除的CAL-27细胞系,分别采用CCK-8、流式细胞术和Transwell方法检测Linc-ROR对OSCC细胞增殖、凋亡以及迁移侵袭能力的影响。采用Western Blot的方法检测Linc-ROR对PI3K/AKT通路的影响。结果与正常的口腔黏膜组织和细胞相比,Linc-ROR表达在OSCC组织和细胞中均显著上调(P<0.05);与NC组相比,过表达Linc-ROR OSC-4细胞的增殖和侵袭能力得到显著升高,凋亡率显著下降(P<0.05);与Sh-NC组相比,敲除Linc-ROR CAL-27细胞的增殖和侵袭能力受到显著抑制,同时凋亡率显著增加(P<0.05);Linc-ROR过表达显著激活PI3K/AKT通路,而Linc-ROR敲除则抑制PI3K和AKT的磷酸化水平。结论 Linc-ROR在OSCC组织和细胞中均显著高表达;Linc-ROR过表达可明显促进OSCC细胞的增殖侵袭及抑制凋亡,作用机制是激活PI3K/AKT通路。

Objective To investigate the Linc-RORexpressionin OSCC tissues and cells and further investigate the regulatory role of Linc-ROR in OSCC cell proliferation and invasion.Methods Tissue sample of patients with OSCC(OSCC group,n=90)wereselected in this study and the relative expression of Linc-ROR in OSCC tissues and cells was analyzed by real-time fluorescence quantitative PCR.The OSC-4 cell lines withLinc-ROR overexpression and CAL-27 cell lines with Linc-ROR knockdown were constructed.The effects of Linc-ROR on proliferation,apoptosis,migration and invasion of OSCC cells were detected by CCK-8,flow cytometry and transwell methods,respectively.The effect of Linc-ROR on PI3K/AKT pathway was detected by western blot.Results The linc-ROR expression was significantly higher in OSCC tissues and cells than that in normal oral mucosa tissues and cells(P<0.05).Besides,overexpressed linc-ROR could significantly promote the prolifera-tion and invasion and sharply suppress the apoptosis of OSC-4 cells when compared with NC group(P<0.05).Interestingly,the effect of Linc-ROR knockdown exhibited the reverse results.What's more,up-regulation of Linc-ROR activated the PI3K/AKT pathway,while knockdown of linc-ror inhibited the phosphorylation of PI3K and AKT.Conclusion Linc-ROR is highly expressed in OSCC tissues and cells.Overexpression of linc-ROR could significantly promote proliferation and inva-sion of OSCC cells and inhibit apoptosis of OSCC cells by activating the PI3K/AKT pathway.