肝细胞生长因子及RECK在胃癌组织中的表达及临床意义

The expression and clinical significance of hepatocyte growth factor and RECK in gastric cancer

目的观察肝细胞生长因子(HGF)及Kazal基序富含半胱氨酸的逆转诱导蛋白(RECK)在胃癌组织中表达情况及其在胃癌发生发展中的作用。方法收集2013年5月—2016年11月石家庄市人民医院普外科确诊胃癌患者60例手术标本,采用免疫组化方法检测手术切除的胃癌组织及相应的癌旁正常组织中HGF及RECK的表达情况,分析两者的表达与临床病理参数的关系及其在胃癌组织中表达的相关性,探讨其与患者预后的关系。结果胃癌组织中HGF阳性表达显著高于癌旁正常组织(80.0%vs.56.7%,χ^2=7.548,P<0.01);胃癌组织中RECK阳性表达显著低于癌旁正常组织(58.3%vs.91.7%,χ^2=17.778,P<0.01);胃癌组织中HGF阳性表达与肿瘤分化程度、TNM分期及淋巴结转移显著相关(P<0.05),而与患者性别、年龄、肿瘤直径及T分期无关(P>0.05)。胃癌组织中RECK阳性表达与T分期、TNM分期及淋巴结转移显著相关(P<0.05),而与患者性别、年龄、肿瘤直径及肿瘤分化程度无关(P>0.05)。HGF阳性亚组术后3年无瘤生存率低于HGF阴性亚组(χ^2=5.889,P<0.05)。在胃癌组织中HGF与RECK阳性表达呈负相关(r=-0.423,P<0.05)。结论胃癌组织中HGF阳性表达水平较高而RECK的表达水平较低,HGF阳性表达的患者预后多不佳,HGF及RECK可能参与了胃癌的侵袭和转移过程。

Objective To observe the expression of hepatocyte growth factor(HGF)and Kazal motif cysteine-rich reverse inducer protein(RECK)in gastric cancer tissues and their role in the development of gastric cancer.Methods Collected 60 surgical specimens of gastric cancer patients diagnosed in General Surgery Department of Shijiazhuang City People’s Hospital from May 2013 to November 2016.Immunohistochemistry was used to detect the expression of HGF and RECK in the surgically resected gastric cancer tissue and the corresponding normal tissues adjacent to the cancer.Analyze the relationship between their expressions and clinicopathological parameters and their correlation in gastric cancer tissues,and explore their relationship with the prognosis of patients.Result The positive expression of HGF in gastric cancer tissue was significantly higher than that in normal tissue adjacent to cancer(80.0%vs.56.7%,χ^2=7.548,P<0.01);the positive expression of RECK in gastric cancer tissue was significantly lower than that in normal tissue adjacent to cancer(χ^2=17.778,P<0.01);The positive expression of HGF in gastric cancer tissue was significantly related to the degree of tumor differentiation,TNM stage and lymph node metastasis(P<0.05),but not to the patient's gender,age,tumor diameter and T stage(P>0.05).The positive expression of RECK in gastric cancer tissue was significantly correlated with T stage,TNM stage and lymph node metastasis(P<0.05),but was not related to the patient's gender,age,tumor diameter and tumor differentiation(P>0.05).The 3-year tumor-free survival rate of the HGF-positive subgroup was lower than that of the HGF-negative group(χ^2=5.889,P<0.05).There was a negative correlation between the positive expression of HGF and RECK in gastric cancer tissues(r=-0.423,P<0.05).Conclusion The positive expression level of HGF in gastric cancer tissues is higher while the expression level of RECK is lower.Patients with positive expression of HGF have poor prognosis.HGF and RECK may be involved in the invasion and metastasis of gastric cancer.