IL-17A可以促进卵巢癌的腹腔转移

IL-17A promotes intraperitoneal metastasis of ovarian cancer

目的:探讨白细胞介素(IL)-17A对卵巢癌(OvCa)腹腔转移的影响。方法:以C57BL/6为遗传背景的野生型(WT)小鼠和IL-17A缺陷(IL-17A-/-)小鼠为研究对象,腹腔注射同基因小鼠OvCa细胞系ID8,检测内源性IL-17A对OvCa腹腔种植瘤生长的影响。应用MTT法检测不同浓度的重组小鼠IL-17A(rmIL-17A)对ID8细胞增殖的影响;应用划痕实验检测不同浓度的rmIL-17A对ID8细胞迁移能力的影响;应用Transwell侵袭实验检测不同浓度的rmIL-17A对ID8细胞侵袭能力的影响。结果:与IL-17A-/-小鼠相比,WT小鼠腹腔内肠系膜、腹膜后淋巴结、脾脏和肾脏表面等处均见密集瘤结节转移灶,且腹腔内瘤结节数量显著增多(均P<0.01)。体外实验结果显示,与对照组相比,不同浓度的rmIL-17A对ID8细胞增殖无影响(均P>0.05);与对照组相比,10 ng/mL的rmIL-17A作用6 h后可促进ID8细胞迁移,1 ng/mL和10 ng/mL的rmIL-17A作用12 h后可显著促进ID8细胞迁移(均P<0.05);与对照组相比,干预24 h后,1 ng/mL和10 ng/mL的rmIL-17A对ID8细胞的侵袭能力均有促进作用(均P<0.05)。结论:IL-17A可以促进卵巢癌的腹腔转移。

Objective: To investigate the effect of interleukin-17 A(IL-17 A)on intraperitoneal metastasis of ovarian cancer(OvCa).Methods: Wild-type(WT) mice and IL-17 A-deficient(IL-17 A-/-) mice with C57 BL/6 genetic background were used as research subjects.The effect of endogenous IL-17 A on OvCa intraperitoneal metastasis was evaluated by intraperitoneal injection of syngeneic mouse ovarian cancer cell line ID8. MTT assay was used to detect the effect of recombinant mouse IL-17 A(rmIL-17 A) on ID8 cells proliferation. Wound healing assay was used to detect the effect of rmIL-17 A on ID8 cells migration. Transwell invasion assay was used to detect the effect of rmIL-17 A on the invasion ability of ID8 cells. Results: Compared with IL-17 A-/-mice, much denser tumor nodules were observed on the surface of mesentery, retroperitoneal lymph nodes, spleen and kidney in WT mice, and the number of nodules in the abdominal cavity was significantly increased(all P<0.01). In vitro results showed that rmIL-17 A had no effect on ID8 cells proliferation. Compared with control group, rmIL-17 A at 10 ng/mL could promote the migration of ID8 cells after 6 h, and rmIL-17 A at 1 ng/mL and 10 ng/mL could significantly promote the migration of ID8 cells after 12 h(all P<0.05). Compared with control group, after 24 hours of intervention, 1 ng/mL and 10 ng/mL rmIL-17 A both promoted the invasion of ID8 cells(all P<0.05). Conclusion: IL-17 A can enhance the intraperitoneal metastasis of OvCa.